Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells

Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of...

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Published inBiochemical and biophysical research communications Vol. 487; no. 2; pp. 344 - 350
Main Authors Hirano, Kunio, Konagaya, Shuhei, Turner, Alexander, Noda, Yuichiro, Kitamura, Shigeru, Kotera, Hidetoshi, Iwata, Hiroo
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 27.05.2017
Subjects
60 APPLIED LIFE SCIENCES
Batch Cell Culture Techniques - instrumentation
Batch Cell Culture Techniques - methods
cell aggregates
Cell Differentiation - physiology
Cell Separation - instrumentation
Cell Separation - methods
Cells, Cultured
Cellular aggregates
Cellular Reprogramming Techniques - instrumentation
Cellular Reprogramming Techniques - methods
CONTROL SYSTEMS
Equipment Design
Equipment Failure Analysis
Humans
Induced differentiation
iPS cells
islets of Langerhans
Islets of Langerhans - cytology
Lab-On-A-Chip Devices
MATHEMATICAL SOLUTIONS
medicine
methodology
microbial contamination
Microfluidics
Microwells
Pancreatic islet cells
Pluripotent Stem Cells - cytology
somatic cells
STEM CELLS
Microwells
Induced differentiation
Pancreatic islet cells
Cellular aggregates
Microfluidics
iPS cells
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Abstract Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality. •A simple, closed-channel-based, semi-automatic culture system is proposed.•Uniform cell aggregate formation and culture is realized in microwell structure.•Functional islet cells are successfully induced following 30-plus-day protocol.•System requires no daily medium replacement and reduces contamination risk.
AbstractList Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality.
Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality. •A simple, closed-channel-based, semi-automatic culture system is proposed.•Uniform cell aggregate formation and culture is realized in microwell structure.•Functional islet cells are successfully induced following 30-plus-day protocol.•System requires no daily medium replacement and reduces contamination risk.
Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality. - Highlights: • A simple, closed-channel-based, semi-automatic culture system is proposed. • Uniform cell aggregate formation and culture is realized in microwell structure. • Functional islet cells are successfully induced following 30-plus-day protocol. • System requires no daily medium replacement and reduces contamination risk.
Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality.Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality.
Author Kotera, Hidetoshi
Hirano, Kunio
Noda, Yuichiro
Iwata, Hiroo
Konagaya, Shuhei
Kitamura, Shigeru
Turner, Alexander
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  organization: Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogo-in, Sakyo-ku, Kyoto, 606-8507, Japan
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Issue 2
Keywords Microwells
Induced differentiation
Pancreatic islet cells
Cellular aggregates
Microfluidics
iPS cells
Language English
License This is an open access article under the CC BY license.
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Snippet Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative...
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SubjectTerms 60 APPLIED LIFE SCIENCES
Batch Cell Culture Techniques - instrumentation
Batch Cell Culture Techniques - methods
cell aggregates
Cell Differentiation - physiology
Cell Separation - instrumentation
Cell Separation - methods
Cells, Cultured
Cellular aggregates
Cellular Reprogramming Techniques - instrumentation
Cellular Reprogramming Techniques - methods
CONTROL SYSTEMS
Equipment Design
Equipment Failure Analysis
Humans
Induced differentiation
iPS cells
islets of Langerhans
Islets of Langerhans - cytology
Lab-On-A-Chip Devices
MATHEMATICAL SOLUTIONS
medicine
methodology
microbial contamination
Microfluidics
Microwells
Pancreatic islet cells
Pluripotent Stem Cells - cytology
somatic cells
STEM CELLS
Title Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells
URI https://dx.doi.org/10.1016/j.bbrc.2017.04.062
https://www.ncbi.nlm.nih.gov/pubmed/28412348
https://www.proquest.com/docview/1888961617
https://www.proquest.com/docview/2000370003
https://www.osti.gov/biblio/22697032
Volume 487
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