Genetic Heterogeneity of Alicyclobacillus Strains Revealed by RFLP Analysis of vdc Region and rpoB Gene

• Published in: BioMed research international Vol. 2018; no. 2018; pp. 1 - 12
• Main Authors: Dekowska, Agnieszka, Sokołowska, Barbara, Niezgoda, Jolanta
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2018; Hindawi; John Wiley & Sons, Inc
• Subjects: Acids; Alicyclobacillus - genetics; Alicyclobacillus - isolation & purification; Alicyclobacillus - pathogenicity; Analysis; Apple; Bacteria; Beverages; Cluster analysis; DNA, Bacterial - genetics; DNA-Directed RNA Polymerases - genetics; E coli; Food; Food Contamination - analysis; Food Microbiology; Fourier transforms; Fruit - microbiology; Fruit and Vegetable Juices - microbiology; Fruit juices; Genes; Genetic Heterogeneity; Genomes; Genotype; Heat resistance; Heterogeneity; Humans; Identification; Intermediates; Malus - microbiology; Methods; Microbiology; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Restriction fragment length polymorphism; RNA polymerase; RNA, Ribosomal, 16S - genetics; RpoB protein; rRNA 16S; Species; Species Specificity; Spoilage; Australia

PCR-RFLP targeting of the 16S rDNA and rpoB genes, as well as the vdc region, was applied to identify and differentiate between the spoilage and non-spoilage Alicyclobacillus species. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates,...