Biotransformation of mogrosides from Siraitia grosvenorii by Ganoderma lucidum mycelium and the purification of mogroside III E by macroporous resins

• Published in: Yàowu shi͡p︡in fenxi Vol. 28; no. 1; pp. 74 - 83
• Main Authors: Chiu, Chun-Hui, Wang, Reuben, Zhuang, Shasha, Lin, Pei-Yin, Lo, Yi-Chen, Lu, Ting-Jang
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier Taiwan LLC 01.01.2020; Food and Drug Administration
• Subjects: Adsorbents; adsorption; Antifungal agents; Bacteria; Biotransformation; blood glucose; Cancer; Cellobiase; Cellulase; Cucurbitaceae - chemistry; Deglycosylation; desorption; Diaion® HP-20; enzyme activity; Enzymes; Fruit - chemistry; fruit extracts; Fruits; Ganoderma lucidum; Glucosidase; Glucosides - isolation & purification; Glucosides - metabolism; harvesting; Herbal medicine; High-performance liquid chromatography; kinetics; Liquid chromatography; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; Metabolites; Mogroside III E; Mushrooms; Mycelium; Natural & organic foods; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Optimization; porous media; Purification; purification methods; Purity; R&D; Recovery; Reishi - metabolism; Relative abundance; Research & development; Resins; Saponins; Saponins - metabolism; Siraitia grosvenorii; Sugar; Sweetener; Sweeteners; Sweetening Agents; Sweetness; Triterpenes - isolation & purification; Triterpenes - metabolism; β-Glucosidase; St Louis Missouri; United States > US; Taiwan; Ganoderma lucidum; Sweetener; Mogroside III E; Biotransformation; Diaion® HP-20
• DOI: 10.1016/j.jfda.2019.05.001
• ISSN: 1021-9498

Mogrosides are the major triterpenoidal saponins found in swingle, the fruit of Siraitia grosvenorii, which have recently been widely used throughout the world as natural food sweeteners. Among this class of compounds, mogroside III E (MG III E) exhibits the most intense sweetness, and it was also found to effectively regulate blood glucose levels. However, the relative abundance of naturally occurring MG III E is low compared to other mogrosides. Therefore, the purpose of this study was to enrich MG III E through biotransformation of fruit extracts and to develop a reliable method for its purification. We used HPLC coupled with mass spectrometry and nuclear magnetic resonance spectroscopy for metabolite analysis and identified MG III E as a major metabolite of Ganoderma lucidum mycelium. This organism converts the most abundant mogroside, mogroside V, to MG III E via a deglycosylation reaction; high levels of β-glucosidase activities were also detected. In addition, we established an efficient purification method for MG III E using HP-20 macroporous resin. Optimization of the method was accomplished by kinetic model fitting, dynamic adsorption studies, and desorption experiments. The purity of MG III E was increased from 11.71% to 54.19%, with a 70%–76% recovery rate, and the scaled-up purification process allowed us to harvest 17.38 g of MG III E with 55.14% purity and a 74.71% of recovery rate. Therefore, our low cost, time-saving, easy to scale-up procedure for isolating MG III E could be applicable in industrial processes. [Display omitted] •The first paper providing processes for the preparation of Mogroside III E from a biotransformed mogroside mixtures.•Mogroside III E is identified as the major metabolite of the Ganoderma lucidum mycelium during mogrosides biotransformation.•The one step and one column separation technique provide a five-fold increase in the purification factor from 11.7% to 55.1%.