Antibodies to the Epidermal Growth Factor Receptor Block the Biological Activities of Sarcoma Growth Factor

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 80; no. 18; pp. 5627 - 5630
• Main Authors: Carpenter, Graham, Stoscheck, Christa M., Preston, Yvette A., DeLarco, Joseph E.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.09.1983; National Acad Sciences
• Subjects: 3T3 cells; Animals; Antibodies; Antibodies - immunology; Antiserum; Cell Division; Cell growth; Cell lines; Cells; DNA; DNA Replication - drug effects; ErbB Receptors; Fibroblasts - cytology; Humans; Kidney cells; Mice; Peptides - antagonists & inhibitors; Receptors; Receptors, Cell Surface - immunology; Sarcoma; Sarcoma Viruses, Murine; Transforming Growth Factors
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.80.18.5627

The role of the epidermal growth factor (EGF) receptor system in mediating the biological activities of sarcoma growth factor (SGF) has been assessed by using specific anti-EGF receptor antibodies. There are two classes of anti-EGF receptor antibodies, those that block binding of125I-labeled EGF (125I-EGF) and those that do not block binding but do interact with a portion of the EGF receptor on the surface of intact cells. Antisera of both types have been assayed for their capacity to affect the biological activities of SGF. The antisera that block125I-EGF binding to its receptor block the induction of DNA synthesis in human fibroblasts by either EGF or SGF but not by other polypeptide mitogens. Titration of the anti-EGF receptor antiserum indicates the presence of one population of antibody that blocks the site of both EGF and SGF action. Antisera to the EGF receptor that block125I-EGF binding also inhibited the SGF-dependent anchorage-independent growth of normal cells in soft agar. The antisera to the EGF receptor that does not block125-EGF binding or EGF activity did not inhibit any of the biological activities of SGF. The results suggest that occupation of the EGF receptor is required for both the mitogenic and colony-forming activity of SGF.