Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes

Glycans play a fundamental role in several biological processes, such as cell–cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood...

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Published inMetabolites Vol. 11; no. 9; p. 566
Main Authors Ragusa, Andrea, Romano, Pietrina, Lenucci, Marcello Salvatore, Civino, Emanuela, Vergara, Daniele, Pitotti, Elena, Neglia, Cosimo, Distante, Alessandro, Romano, Giampiero Diego, Di Renzo, Nicola, Surico, Giammarco, Piscitelli, Prisco, Maffia, Michele
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 24.08.2021
MDPI
Subjects
Anion-exchange chromatography
Biomarkers
Biopsy
Body mass index
Breast cancer
Cancer therapies
Carbohydrates
Cell adhesion
Chromatography
Clinical medicine
D-Galactosamine
early diagnosis
Galactose
Glucosamine
glycomics
glycoprotein
Glycoproteins
Glycosylation
HPEAC-PAD
Lung cancer
Mannose
Medical prognosis
Mortality
Patients
Polysaccharides
Proteins
Saliva
Statistical analysis
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Abstract Glycans play a fundamental role in several biological processes, such as cell–cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood are currently used in clinical practice as cancer biomarkers (e.g., CA125, PSA, and CA19-9). The development of novel non-invasive diagnostic procedures would greatly simplify the screening and discovery of pathologies at an early stage, thus also allowing for simpler treatment and a higher success rate. In this observational study carried out on 68 subjects diagnosed with either breast or lung cancer and 34 healthy volunteers, we hydrolyzed the glycoproteins in saliva and quantified the obtained free sugars (fucose, mannose, galactose, glucosamine, and galactosamine) by using high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD). The glycosidic profiles were compared by using multivariate statistical analysis, showing differential glycosylation patterns among the three categories. Furthermore, Receiver Operating Characteristics (ROC) analysis allowed obtaining a reliable and minimally invasive protocol able to discriminate between healthy and pathological subjects.
AbstractList Glycans play a fundamental role in several biological processes, such as cell–cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood are currently used in clinical practice as cancer biomarkers (e.g., CA125, PSA, and CA19-9). The development of novel non-invasive diagnostic procedures would greatly simplify the screening and discovery of pathologies at an early stage, thus also allowing for simpler treatment and a higher success rate. In this observational study carried out on 68 subjects diagnosed with either breast or lung cancer and 34 healthy volunteers, we hydrolyzed the glycoproteins in saliva and quantified the obtained free sugars (fucose, mannose, galactose, glucosamine, and galactosamine) by using high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD). The glycosidic profiles were compared by using multivariate statistical analysis, showing differential glycosylation patterns among the three categories. Furthermore, Receiver Operating Characteristics (ROC) analysis allowed obtaining a reliable and minimally invasive protocol able to discriminate between healthy and pathological subjects.
Glycans play a fundamental role in several biological processes, such as cell-cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood are currently used in clinical practice as cancer biomarkers (e.g., CA125, PSA, and CA19-9). The development of novel non-invasive diagnostic procedures would greatly simplify the screening and discovery of pathologies at an early stage, thus also allowing for simpler treatment and a higher success rate. In this observational study carried out on 68 subjects diagnosed with either breast or lung cancer and 34 healthy volunteers, we hydrolyzed the glycoproteins in saliva and quantified the obtained free sugars (fucose, mannose, galactose, glucosamine, and galactosamine) by using high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD). The glycosidic profiles were compared by using multivariate statistical analysis, showing differential glycosylation patterns among the three categories. Furthermore, Receiver Operating Characteristics (ROC) analysis allowed obtaining a reliable and minimally invasive protocol able to discriminate between healthy and pathological subjects.Glycans play a fundamental role in several biological processes, such as cell-cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood are currently used in clinical practice as cancer biomarkers (e.g., CA125, PSA, and CA19-9). The development of novel non-invasive diagnostic procedures would greatly simplify the screening and discovery of pathologies at an early stage, thus also allowing for simpler treatment and a higher success rate. In this observational study carried out on 68 subjects diagnosed with either breast or lung cancer and 34 healthy volunteers, we hydrolyzed the glycoproteins in saliva and quantified the obtained free sugars (fucose, mannose, galactose, glucosamine, and galactosamine) by using high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD). The glycosidic profiles were compared by using multivariate statistical analysis, showing differential glycosylation patterns among the three categories. Furthermore, Receiver Operating Characteristics (ROC) analysis allowed obtaining a reliable and minimally invasive protocol able to discriminate between healthy and pathological subjects.
Author Romano, Pietrina
Maffia, Michele
Di Renzo, Nicola
Neglia, Cosimo
Ragusa, Andrea
Piscitelli, Prisco
Lenucci, Marcello Salvatore
Pitotti, Elena
Surico, Giammarco
Civino, Emanuela
Vergara, Daniele
Distante, Alessandro
Romano, Giampiero Diego
AuthorAffiliation 3 Medical Oncology Unit, “Vito Fazzi” Hospital, 73100 Lecce, Italy; info@isbem.it (P.R.); oncologia.polecce@ausl.le.it (G.D.R.); direnzo.ematolecce@gmail.com (N.D.R.); repol@ausl.le.it (G.S.)
1 Department of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100 Lecce, Italy; marcello.lenucci@unisalento.it (M.S.L.); emanuela.civino@unisalento.it (E.C.); daniele.vergara@unisalento.it (D.V.)
2 CNR-Nanotec, Institute of Nanotechnology, via Monteroni, 73100 Lecce, Italy
5 Euro Mediterranean Scientific Biomedical Institute (ISBEM), 72100 Brindisi, Italy; neglia@isbem.it (C.N.); distante@isbem.it (A.D.)
4 Local Health Authority ASL Lecce, 73100 Lecce, Italy; proteomica.polecce@ausl.le.it (E.P.); piscitelli@unescochairnapoli.it (P.P.)
AuthorAffiliation_xml – name: 4 Local Health Authority ASL Lecce, 73100 Lecce, Italy; proteomica.polecce@ausl.le.it (E.P.); piscitelli@unescochairnapoli.it (P.P.)
– name: 5 Euro Mediterranean Scientific Biomedical Institute (ISBEM), 72100 Brindisi, Italy; neglia@isbem.it (C.N.); distante@isbem.it (A.D.)
– name: 2 CNR-Nanotec, Institute of Nanotechnology, via Monteroni, 73100 Lecce, Italy
– name: 3 Medical Oncology Unit, “Vito Fazzi” Hospital, 73100 Lecce, Italy; info@isbem.it (P.R.); oncologia.polecce@ausl.le.it (G.D.R.); direnzo.ematolecce@gmail.com (N.D.R.); repol@ausl.le.it (G.S.)
– name: 1 Department of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100 Lecce, Italy; marcello.lenucci@unisalento.it (M.S.L.); emanuela.civino@unisalento.it (E.C.); daniele.vergara@unisalento.it (D.V.)
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Snippet Glycans play a fundamental role in several biological processes, such as cell–cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is...
Glycans play a fundamental role in several biological processes, such as cell-cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is...
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StartPage 566
SubjectTerms Anion-exchange chromatography
Biomarkers
Biopsy
Body mass index
Breast cancer
Cancer therapies
Carbohydrates
Cell adhesion
Chromatography
Clinical medicine
D-Galactosamine
early diagnosis
Galactose
Glucosamine
glycomics
glycoprotein
Glycoproteins
Glycosylation
HPEAC-PAD
Lung cancer
Mannose
Medical prognosis
Mortality
Patients
Polysaccharides
Proteins
Saliva
Statistical analysis
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Title Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes
URI https://www.proquest.com/docview/2576437158
https://www.proquest.com/docview/2576904874
https://pubmed.ncbi.nlm.nih.gov/PMC8471868
https://doaj.org/article/e80b1748ffcb4e6f9566366722483936
Volume 11
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