Releasing iron from ferritin protein nanocage by reductive method: The role of electron transfer mediator

• Published in: Biochimica et biophysica acta. General subjects Vol. 1862; no. 5; pp. 1190 - 1198
• Main Authors: Koochana, Prashanth Kumar, Mohanty, Abhinav, Das, Suman, Subhadarshanee, Biswamaitree, Satpati, Suresh, Dixit, Anshuman, Sabat, Surendra Chandra, Behera, Rabindra K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2018
• Subjects: Amphibian Proteins - chemistry; Amphibian Proteins - metabolism; Animals; Anura; biochemical pathways; Dissolved O2 consumption; dissolved oxygen; Electron relay; electron transfer; Electron Transport; encapsulation; ferric oxide; ferrihydrite; Ferritin; Ferritins - chemistry; Ferritins - metabolism; fluorescence; frogs; iron; Iron - chemistry; Iron - metabolism; Iron release; microbial growth; Mid-point redox potential; Models, Chemical; molecular models; NAD (coenzyme); NAD - chemistry; NAD - metabolism; NADH oxidation; oxidation; Oxidation-Reduction; Oxygen - chemistry; Oxygen - metabolism; oxygen consumption; Pseudomonas aeruginosa; reducing agents; solubility; virulence; MRed; MOPS; Electron relay; FMN; PYO; Ferritin; Iron release; NADH oxidation; ET; E1/2; NADH; Fz; PMS; ROS; MOx; Mid-point redox potential; Dissolved O2 consumption; Dissolved O consumption

Ferritin detoxifies excess of free Fe(II) and concentrates it in the form of ferrihydrite (Fe2O3·xH2O) mineral. When in need, ferritin iron is released for cellular metabolic activities. However, the low solubility of Fe(III) at neutral pH, its encapsulation by stable protein nanocage and presence o...