RT-PCR/MALDI-TOF Diagnostic Target Performance Reflects Circulating SARS-CoV-2 Variant Diversity in New York City

• Published in: The Journal of molecular diagnostics : JMD Vol. 24; no. 7; pp. 738 - 749
• Main Authors: Hernandez, Matthew M., Banu, Radhika, Gonzalez-Reiche, Ana S., Gray, Brandon, Shrestha, Paras, Cao, Liyong, Chen, Feng, Shi, Huanzhi, Hanna, Ayman, Ramírez, Juan David, van de Guchte, Adriana, Sebra, Robert, Gitman, Melissa R., Nowak, Michael D., Cordon-Cardo, Carlos, Schutzbank, Ted E., Simon, Viviana, van Bakel, Harm, Sordillo, Emilia Mia, Paniz-Mondolfi, Alberto E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2022; American Society for Investigative Pathology
• Subjects: COVID-19 - diagnosis; COVID-19 - epidemiology; Humans; New York City - epidemiology; Regular; Reverse Transcriptase Polymerase Chain Reaction; SARS-CoV-2 - genetics; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; New York City

As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to circulate, multiple variants of concern have emerged. New variants pose challenges for diagnostic platforms because sequence diversity can alter primer/probe-binding sites (PBSs), causing false-negative results. The MassARR...