Fluorescence microscopy for visualizing single-molecule protein dynamics

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 2; p. 129362
• Main Author: Yokota, Hiroaki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2020
• Subjects: fluorescence; fluorescence microscopy; fluorescent dyes; Fluorescent nanodiamond (FND); image analysis; nanodiamonds; Optically detected magnetic resonance (ODMR); photostability; Protein dynamics; Single-molecule fluorescence imaging (smFI); Super-resolution microscopy; MOPS; MPC; ZMWs; Fluorescent nanodiamond (FND); AFM; smFRET; EMCCD; NBA; TIRF; 2D; DDS; PALM; NHS; ODMR; Super-resolution microscopy; SSIM; Optically detected magnetic resonance (ODMR); FIONA; DOPI; STED; smFI; COT; Protein dynamics; Single-molecule fluorescence imaging (smFI); PCA; PCD; Qrod; STORM; 3D; PEG; FND; Qdot

Single-molecule fluorescence imaging (smFI) has evolved into a valuable method used in biophysical and biochemical studies as it can observe the real-time behavior of individual protein molecules, enabling understanding of their detailed dynamic features. smFI is also closely related to other state-...