Detection of urinary microRNA biomarkers using diazo sulfonamide-modified screen printed carbon electrodes

• Published in: RSC advances Vol. 11; no. 31; pp. 18832 - 18839
• Main Authors: Smith, Daniel A, Simpson, Kate, Lo Cicero, Matteo, Newbury, Lucy J, Nicholas, Philip, Fraser, Donald J, Caiger, Nigel, Redman, James E, Bowen, Timothy
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 25.05.2021; The Royal Society of Chemistry
• Subjects: Biomarkers; Biosensors; Carbon; Chemistry; detection limit; diazo compounds; Disease control; Disorders; Electrochemical analysis; electrochemistry; humans; Kidney diseases; liquids; microRNA; MicroRNAs; Ribonucleic acid; RNA; Sulfonamides; urine

This paper describes a straightforward electrochemical method for rapid and robust urinary microRNA (miRNA) quantification using disposable biosensors that can discriminate between urine from diabetic kidney disease (DKD) patients and control subjects. Aberrant miRNA expression has been observed in...