Role and mechanism of homocysteine in affecting hepatic protein-tyrosine phosphatase 1B

• Published in: Biochimica et biophysica acta. General subjects Vol. 1863; no. 5; pp. 941 - 949
• Main Authors: Liu, Ping, Huang, Jin, Zhong, Liangwei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2019
• Subjects: glucose; hepatocytes; Homocysteine; insulin; insulin receptors; Insulin signaling; liquid chromatography; NADP (coenzyme); phosphorylation; precipitin tests; PTP1B; RNA interference; S-homocysteinylation; tandem mass spectrometry; thiols; Thioredoxin system; thioredoxins; Western blotting; HSSH; IRβ; pNPP; TrxR1; Trx1; PTP1B; Thioredoxin system; IAM; PMSF; 5-IAF; HcySH; NEM; S-Hcy-PTP1B; Homocysteine; Insulin signaling; S-homocysteinylation

Elevated homocysteine is epidemiologically related to insulin resistance. Protein-tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin signaling. However, the effect of homocysteine on PTP1B remains unclear. S-homocysteinylated PTP1B was identified by LC-ESI-MS/MS. The ability of thior...