Defining Regulatory Regions in the Rat Prolactin Gene Family Locus Using a Large P1 Genomic Clone

Members of the large rat prolactin gene family located on chromosome 17 are expressed in one or more placental trophoblast cell types and maternal decidua at specific times during pregnancy. Studies to identify the factors involved in these highly specific developmental expression patterns, using li...

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Published inEndocrinology (Philadelphia) Vol. 144; no. 11; pp. 4742 - 4754
Main Authors Öztürk, Arzu, Fresnoza, Agnes, Savoie, Amanda, Duckworth, Harry W, Duckworth, Mary Lynn
Format Journal Article
LanguageEnglish
Published Bethesda, MD Endocrine Society 01.11.2003
Oxford University Press
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Abstract Members of the large rat prolactin gene family located on chromosome 17 are expressed in one or more placental trophoblast cell types and maternal decidua at specific times during pregnancy. Studies to identify the factors involved in these highly specific developmental expression patterns, using limited amounts of 5′-flanking DNA, have met with only partial success. Here we report the isolation and characterization of an 80-kb rat genomic clone, P1 12830, containing linked rat placental lactogen II, rat prolactin-like protein-I, and rat prolactin-like protein-B genes with substantial amounts of 5′- and 3′-flanking DNA as well as a rat placental lactogen II-related pseudogene, the first to be described in this gene family. This clone was used to create F0 transgenic mice, and the levels of expression of the three rat genes were compared with those of the endogenous mouse genes, using RT-PCR. Each rat gene was expressed differently in the same placenta, confirming the importance of sufficient flanking sequences in the expression of the individual genes. These studies emphasize the need for large genomic clones in defining the complete complement of factors that regulate the developmental expression of the rat prolactin gene locus.
AbstractList Members of the large rat prolactin gene family located on chromosome 17 are expressed in one or more placental trophoblast cell types and maternal decidua at specific times during pregnancy. Studies to identify the factors involved in these highly specific developmental expression patterns, using limited amounts of 5′-flanking DNA, have met with only partial success. Here we report the isolation and characterization of an 80-kb rat genomic clone, P1 12830, containing linked rat placental lactogen II, rat prolactin-like protein-I, and rat prolactin-like protein-B genes with substantial amounts of 5′- and 3′-flanking DNA as well as a rat placental lactogen II-related pseudogene, the first to be described in this gene family. This clone was used to create F0 transgenic mice, and the levels of expression of the three rat genes were compared with those of the endogenous mouse genes, using RT-PCR. Each rat gene was expressed differently in the same placenta, confirming the importance of sufficient flanking sequences in the expression of the individual genes. These studies emphasize the need for large genomic clones in defining the complete complement of factors that regulate the developmental expression of the rat prolactin gene locus.
Author Duckworth, Mary Lynn
Savoie, Amanda
Duckworth, Harry W
Öztürk, Arzu
Fresnoza, Agnes
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Issue 11
Keywords Vertebrata
Mammalia
Adenohypophyseal hormone
Gene
Rat
Family study
Prolactin
Animal
Rodentia
Regulatory sequence
Locus
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Snippet Members of the large rat prolactin gene family located on chromosome 17 are expressed in one or more placental trophoblast cell types and maternal decidua at...
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StartPage 4742
SubjectTerms Amino Acid Sequence
Animals
Biological and medical sciences
Chromosome 17
Chromosome Mapping
Cloning
Cloning, Molecular
Decidua
Deoxyribonucleic acid
DNA
Feasibility Studies
Female
Fundamental and applied biological sciences. Psychology
Gene Expression
Gene sequencing
Genes
Genome
Genomics
Mice
Mice, Inbred Strains
Mice, Transgenic
Molecular Sequence Data
Multigene Family - genetics
Nucleotide sequence
Placenta
Placenta - metabolism
Placental Lactogen - genetics
Pregnancy
Prolactin
Prolactin - genetics
Proteins
Pseudogenes
Rats
Regulatory sequences
Regulatory Sequences, Nucleic Acid
Sequence Analysis, DNA
Transgenes
Transgenic mice
Vertebrates: endocrinology
Title Defining Regulatory Regions in the Rat Prolactin Gene Family Locus Using a Large P1 Genomic Clone
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