An AT-Rich Sequence in Human Common Fragile Site FRA16D Causes Fork Stalling and Chromosome Breakage in S. cerevisiae

• Published in: Molecular cell Vol. 27; no. 3; pp. 367 - 379
• Main Authors: Zhang, Haihua, Freudenreich, Catherine H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.08.2007
• Subjects: AT Rich Sequence - genetics; Base Pairing; Base Sequence; Chromosome Breakage; Chromosome Fragile Sites; Chromosomes, Human, Pair 16 - genetics; Cytogenetic Analysis; DNA; Gene Expression Regulation; Humans; HUMDISEASE; Molecular Sequence Data; Nucleic Acid Conformation; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; DNA; HUMDISEASE

Common fragile sites are regions of human chromosomes prone to breakage. Fragile site FRA16D spans the WWOX/FOR tumor suppressor gene and has been linked to cancer-causing deletions and translocations. Using a genetic assay in yeast, we found that a short AT-rich region (Flex1) within FRA16D increas...