Single cell network profiling (SCNP): mapping drug and target interactions

• Published in: Assay and drug development technologies Vol. 8; no. 3; p. 321
• Main Authors: Covey, Todd M, Putta, Santosh, Cesano, Alessandra
• Format: Journal Article
• Language: English
• Published: United States 01.06.2010
• Subjects: Algorithms; Antineoplastic Agents - pharmacology; Blood Cells - drug effects; Cells - drug effects; Confidence Intervals; Drug Delivery Systems - methods; Drug Evaluation, Preclinical - methods; Drug Industry; Enzyme Inhibitors - pharmacology; Humans; In Vitro Techniques; Janus Kinases - metabolism; Phosphatidylinositol 3-Kinases - antagonists & inhibitors; Phosphorylation; Protein Kinase Inhibitors - pharmacology; Reproducibility of Results; Signal Transduction - drug effects; Small Molecule Libraries; Staining and Labeling; STAT1 Transcription Factor - metabolism; STAT5 Transcription Factor - metabolism

Measuring target coverage of small molecule inhibitors is paramount-first, for selection of molecules to progress through the drug development process and second, once a candidate drug moves to clinical testing, for guiding dose/schedule selection. Single cell network profiling (SCNP) using multipar...