A high-throughput assay of NK cell activity in whole blood and its clinical application

• Published in: Biochemical and biophysical research communications Vol. 445; no. 3; pp. 584 - 590
• Main Authors: Lee, Saet-byul, Cha, Junhoe, Kim, Im-kyung, Yoon, Joo Chun, Lee, Hyo Joon, Park, Sang Woo, Cho, Sunjung, Youn, Dong-Ye, Lee, Heyja, Lee, Choong Hwan, Lee, Jae Myun, Lee, Kang Young, Kim, Jongsun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.03.2014
• Subjects: 60 APPLIED LIFE SCIENCES; Adult; Aged; Aged, 80 and over; blood serum; CHROMIUM 51; colorectal neoplasms; Colorectal Neoplasms - blood; Colorectal Neoplasms - immunology; COMPARATIVE EVALUATIONS; CONCENTRATION RATIO; disease surveillance; ELISA; ENZYME IMMUNOASSAY; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; ENZYMES; Female; genetic markers; HAZARDOUS MATERIALS; High-throughput screening; High-Throughput Screening Assays - methods; Humans; IFN-γ; innate immunity; INTERFERON; interferon-gamma; Interferon-gamma - blood; Interferon-gamma - immunology; Killer Cells, Natural - immunology; LYMPHOCYTES; Male; microsatellite repeats; Middle Aged; NATURAL KILLER CELLS; neoplasm cells; NEOPLASMS; NK cell activity; PATIENTS; radionuclides; SCREENING; Simple assay; STIMULATION; TUMOR CELLS; VIRUSES; High-throughput screening; IFN-γ; Simple assay; NK cell activity; ELISA

•We demonstrated a simple assay of NK cell activity from whole blood.•The measurement of secreted IFN-γ from NK cell enables high-throughput screening.•The NKA assay was validated by clinical results of colorectal cancer patients. Natural killer (NK) cells are lymphocytes of the innate immune system...