BRK phosphorylates PSF promoting its cytoplasmic localization and cell cycle arrest

• Published in: Cellular signalling Vol. 21; no. 9; pp. 1415 - 1422
• Main Authors: Lukong, Kiven E., Huot, Marc-Étienne, Richard, Stéphane
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.09.2009
• Subjects: Breast cancer; Breast Neoplasms - metabolism; BRK; Cell Cycle; Cell Line; Cell Movement; Cell Proliferation; Cytoplasm - metabolism; Epidermal growth factor; Epidermal Growth Factor - metabolism; Epidermal Growth Factor - pharmacology; Female; Humans; Mass spectrometry; Neoplasm Proteins - metabolism; Phosphorylation; Protein-Tyrosine Kinases - metabolism; PSF; PTB-Associated Splicing Factor; RNA-binding proteins; RNA-Binding Proteins - metabolism; Signal Transduction; src Homology Domains; Tyrosine kinases; PSF; Epidermal growth factor; Tyrosine kinases; BRK; Breast cancer; RNA-binding proteins; Mass spectrometry
• ISSN: 0898-6568; 1873-3913
• DOI: 10.1016/j.cellsig.2009.04.008

BReast tumor Kinase (BRK) also known as protein kinase 6 (PTK6) is a non-receptor tyrosine kinase overexpressed in the majority of human breast carcinoma. The expression of BRK is a known prognostic marker of breast carcinoma. BRK has been shown to lie downstream of epidermal growth factor (EGF) signaling and mediate effects on cell proliferation and migration. To identify BRK substrates and interacting proteins, we undertook a proteomic approach. BRK immune complexes were purified from the BT-20 breast cancer cell line and analyzed by mass spectrometry. Herein, we report the identification of PSF, the polypyrimidine tract-binding (PTB) protein-associated splicing factor, as a BRK-interacting protein and substrate. BRK and PSF co-eluted in a large protein complex that was regulated by EGF stimulation. Furthermore, BRK and PSF co-immunoprecipitated in BT-20 cells and we defined the interaction as being an SH3 domain–polyproline interaction. The C-terminal tyrosines of PSF were the site of phosphorylation by BRK. Moreover, tyrosine phosphorylation of PSF was also observed upon EGF stimulation, consistent with a role of PSF and BRK downstream of the EGF receptor. Interestingly, the tyrosine phosphorylation promoted the cytoplasmic relocalization of PSF, impaired its binding to polypyrimidine RNA, and led to cell cycle arrest. Our findings show that BRK targets the PSF RNA-binding protein during EGF stimulation.