Mechanisms of γ-glutamylcysteine ligase regulation

• Published in: Biochimica et biophysica acta Vol. 1760; no. 2; pp. 233 - 244
• Main Authors: Toroser, Dikran, Yarian, Connie S., Orr, William C., Sohal, Rajindar S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2006
• Subjects: Adenosine Triphosphate - pharmacology; Allosteric Regulation; Animals; Brain - enzymology; Buthionine Sulfoximine - pharmacology; Chromatography, High Pressure Liquid; Drosophila melanogaster; Enzyme Activation; Glutamate-Cysteine Ligase - antagonists & inhibitors; Glutamate-Cysteine Ligase - metabolism; Glutathione; Hydrogen-Ion Concentration; Kinetics; Liver - enzymology; Male; Mice; Mice, Inbred C57BL; Myocardium - enzymology; NADP - metabolism; Oxidative stress; Phosphorylation; Protein Kinase Inhibitors - pharmacology; Rats; Rats, Inbred F344; Signal Transduction; γ-glutamylcysteine ligase; GSSG; γ-glutamylcysteine ligase; Signal transduction; Oxidative stress; Phosphorylation; ECD; acivicin; γ-GC; l-BSO; GSH; HPLC; Glutathione

The principal objective of this study was to investigate the mechanisms regulating the activity of γ-glutamylcysteine ligase (GCL; EC 6.3.2.2), the rate limiting enzyme in glutathione biosynthesis. Two phylogenetically divergent species, mouse and the fruitfly, Drosophila melanogaster were used to t...