Proteome analysis of chloroplasts from the moss Physcomitrella patens (Hedw.) B.S.G

• Published in: Biochemistry (Moscow) Vol. 75; no. 12; pp. 1470 - 1483
• Main Authors: Polyakov, N. B, Slizhikova, D. K, Izmalkova, M. Yu, Cherepanova, N. I, Kazakov, V. S, Rogova, M. A, Zhukova, N. A, Alexeev, D. G, Bazaleev, N. A, Skripnikov, A. Yu, Govorun, V. M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.12.2010; SP MAIK Nauka/Interperiodica; Springer; Springer Nature B.V
• Subjects: Amino acids; Analysis; Biochemistry; Biodegradation; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Bryopsida - chemistry; Carbohydrates; Chloroplasts; Chloroplasts - chemistry; Databases, Protein; Hormones; Life Sciences; Lipids; Metabolic Networks and Pathways; Microbiology; Models, Biological; Mosses; Peptides; Photosynthesis; Plant Proteins - chemistry; Post-translational modification; Proteins; Proteolysis; Proteome - chemistry; Proteomics; Protoplasts - chemistry; Subcellular Fractions - chemistry; Sulfur; Vitamins; chloroplast; photosynthesis; subcellular localization; proteome

Intact chloroplasts were prepared from protoplasts of the moss Physcomitrella patens according to an especially developed method. They were additionally separated into stroma and thylakoid fractions. The proteomes of intact plastids, stroma, and thylakoids were analyzed by 1D-electrophoresis under d...