Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri, enhances sucrose content in Solanum lycopersicum fruit

• Published in: Plant physiology and biochemistry Vol. 105; pp. 150 - 161
• Main Authors: Wang, Li-Fen, Qi, Xiao-Xiao, Huang, Xiao-San, Xu, Lin-Lin, Jin, Cong, Wu, Jun, Zhang, Shao-Ling
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.08.2016
• Subjects: Amino Acid Sequence; amino acids; Arabidopsis; Cell Membrane - metabolism; Cloning, Molecular; DNA, Complementary - genetics; Flowering; Flowers - physiology; fructose; Fruit - genetics; Fruit - metabolism; fruits; Gene Expression Regulation, Plant; gene overexpression; genes; Genes, Plant; glucose; leaves; Lycopersicon esculentum - anatomy & histology; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; Membrane Transport Proteins - chemistry; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; onions; Pear; pears; Phenotype; Photosynthesis; Photosynthetic rate; Phylogeny; plant growth; Plant Leaves - physiology; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified; plasma membrane; protoplasts; Pyrus - genetics; Pyrus bretschneideri; Real-Time Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA; Solanum lycopersicum; Subcellular Fractions - metabolism; sucrose; Sucrose - metabolism; Sucrose transporter; Sugar content; tomatoes; transgenic plants; Transgenic tomato; transporters; Flowering; Photosynthetic rate; Sucrose transporter; Pear; Sugar content; Transgenic tomato

Sucrose transporters (SUTs) belong to the major facilitator superfamily. The function of SUTs has been intensively investigated in some higher plants, whereas that in pear fruit is unknown. In this study, the cloning and functional characterization of a sucrose transporter, PbSUT2, in pear (Pyrus br...