In situ structural biology using in-cell NMR

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 2; p. 129364
• Main Authors: Nishida, Noritaka, Ito, Yutaka, Shimada, Ichio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2020
• Subjects: bacteria; bioreactors; cellular microenvironment; chemical reactions; culture media; drug interactions; enzymes; eukaryotic cells; functional properties; isotope labeling; monitoring; nuclear magnetic resonance spectroscopy; protein structure; proteins; structural biology

Accumulating evidence from the experimental and computational studies indicated that the functional properties of proteins are different between in vitro and living cells, raising the necessity to examine the protein structure under the native intracellular milieu. To gain structural information of...