Dynamic Rewiring of Promoter-Anchored Chromatin Loops during Adipocyte Differentiation

• Published in: Molecular cell Vol. 66; no. 3; pp. 420 - 435.e5
• Main Authors: Siersbæk, Rasmus, Madsen, Jesper Grud Skat, Javierre, Biola Maria, Nielsen, Ronni, Bagge, Emilie Kristine, Cairns, Jonathan, Wingett, Steven William, Traynor, Sofie, Spivakov, Mikhail, Fraser, Peter, Mandrup, Susanne
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.05.2017
• Subjects: 3T3-L1 Cells; acetylation; adipocyte differentiation; adipocytes; Adipocytes - metabolism; Adipogenesis; Animals; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; ChIP-seq; chromatin; Chromatin - chemistry; Chromatin - genetics; Chromatin - metabolism; Chromatin Assembly and Disassembly; Chromatin Immunoprecipitation; chromatin looping; Chromosomal Proteins, Non-Histone - genetics; Chromosomal Proteins, Non-Histone - metabolism; corepressors; E1A-Associated p300 Protein - genetics; E1A-Associated p300 Protein - metabolism; Enhancer Elements, Genetic; histones; lysine; Mediator Complex Subunit 1 - genetics; Mediator Complex Subunit 1 - metabolism; Mice; Nucleic Acid Conformation; poised enhancers; promoter capture Hi-C; Promoter Regions, Genetic; regulatory sequences; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Analysis, RNA; single nucleotide variance; Structural Maintenance of Chromosome Protein 1; super-enhancers; Time Factors; topologically associating domains; transcription (genetics); Transcription, Genetic; Transcriptional Activation; transcriptional regulation; corepressors; promoter capture Hi-C; chromatin looping; adipocyte differentiation; transcriptional regulation; ChIP-seq; poised enhancers; topologically associating domains; single nucleotide variance; super-enhancers
• ISSN: 1097-2765; 1097-4164; 1097-4164
• DOI: 10.1016/j.molcel.2017.04.010

Interactions between transcriptional promoters and their distal regulatory elements play an important role in transcriptional regulation; however, the extent to which these interactions are subject to rapid modulations in response to signals is unknown. Here, we use promoter capture Hi-C to demonstr...