Reversible assembly and disassembly of V-ATPase during the lysosome regeneration cycle

• Published in: Molecular biology of the cell Vol. 35; no. 5; p. ar63
• Main Authors: Sava, Ioana, Davis, Luther J., Gray, Sally R., Bright, Nicholas A., Luzio, J. Paul
• Format: Journal Article
• Language: English
• Published: United States American Society for Cell Biology 01.05.2024; The American Society for Cell Biology
• Series: Highlights from
• Subjects: Adenosine triphosphatase; Cells; Proteins; Special Issue on Cell Biology of the Lysosome; United States; United Kingdom

Regulation of the luminal pH of late endocytic compartments in continuously fed mammalian cells is poorly understood. Using normal rat kidney fibroblasts, we investigated the reversible assembly/disassembly of the proton pumping V-ATPase when endolysosomes are formed by kissing and fusion of late en...