Direct effects of airborne PM2.5 exposure on macrophage polarizations

• Published in: Biochimica et biophysica acta Vol. 1860; no. 12; pp. 2835 - 2843
• Main Authors: Zhao, Qingjie, Chen, Hui, Yang, Tao, Rui, Wei, Liu, Fang, Zhang, Fang, Zhao, Yong, Ding, Wenjun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2016
• Subjects: air; air pollution; Animals; Bronchoalveolar Lavage Fluid - cytology; Cell Differentiation - drug effects; Cell Survival - drug effects; cell viability; dose response; flow cytometry; gene deletion; Gene Expression Regulation; genetic markers; granulocyte-macrophage colony-stimulating factor; Granulocyte-Macrophage Colony-Stimulating Factor - genetics; Granulocyte-Macrophage Colony-Stimulating Factor - immunology; Inflammation; innate immunity; interleukin-1beta; Interleukin-1beta - genetics; Interleukin-1beta - immunology; interleukin-6; Interleukin-6 - genetics; Interleukin-6 - immunology; lipopolysaccharides; Lipopolysaccharides - pharmacology; Macrophages; Macrophages, Alveolar - cytology; Macrophages, Alveolar - drug effects; Macrophages, Alveolar - immunology; Macrophages, Peritoneal - cytology; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - immunology; Mice; Mice, Inbred C57BL; Mice, Knockout; mitochondria; Mitochondria - drug effects; Particle Size; Particulate Matter - toxicity; particulates; PM2.5; Polarization; pollutants; Primary Cell Culture; quantitative polymerase chain reaction; reactive oxygen species; Reactive Oxygen Species - agonists; Reactive Oxygen Species - immunology; Reactive Oxygen Species - metabolism; ROS; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - immunology; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Polarization; Fizz1; Arg1; M1; M2; Inflammation; MTT; IL-1β; Macrophages; LPS; IL-6; DCFH-DA; NAC; MFI; TNF-α; GM-CSF; ROS; PM2.5; Ym1; PM; PM(2.5)

Exposure of atmospheric particulate matter with an aerodynamic diameter less than 2.5μm (PM2.5) is epidemiologically associated with illnesses. Potential effects of air pollutants on innate immunity have raised concerns. As the first defense line, macrophages are able to induce inflammatory response...