Generation of the heterogeneity of extracellular vesicles by membrane organization and sorting machineries

• Published in: Biochimica et biophysica acta. General subjects Vol. 1863; no. 4; pp. 681 - 691
• Main Authors: Harada, Yoichiro, Suzuki, Takehiro, Fukushige, Tomoko, Kizuka, Yasuhiko, Yagi, Hirokazu, Yamamoto, Mika, Kondo, Kiyotaka, Inoue, Hiromasa, Kato, Koichi, Taniguchi, Naoyuki, Kanekura, Takuro, Dohmae, Naoshi, Maruyama, Ikuro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2019
• Subjects: biogenesis; detergents; ESCRT; Exosomes; Extracellular vesicles; hepatocyte growth factor receptor; Heterogeneity; Membrane microdomains; metalloproteinases; precipitin tests; proteolysis; RNA interference; secretion; solubilization; sucrose; ultracentrifugation; Extracellular vesicles; Heterogeneity; ESCRT; Membrane microdomains; Exosomes

Cells secrete heterogeneous populations of extracellular vesicles (EVs) via unknown mechanisms. EV biogenesis has been postulated to involve lipid-protein clusters, also known as membrane microdomains. Membrane properties and heterogeneity of melanoma-derived EVs were analyzed by a detergent solubil...