Domain selective labeling for NMR studies of multidomain proteins by domain ligation using highly active sortase A

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 2; p. 129419
• Main Authors: Aizu, Takahiro, Suzuki, Takumi, Kido, Akihiro, Nagai, Kan, Kobayashi, Ayaho, Sugiura, Reiko, Ito, Yutaka, Mishima, Masaki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2020
• Subjects: Multidomain protein; Nrd1; nuclear magnetic resonance spectroscopy; protein synthesis; proteins; Sortase A; Vinculin; Sortase A; Multidomain protein; Nrd1; Vinculin

Structural study of multidomain proteins using NMR is an emerging issue for understanding biological functions. To this end, domain-specific labeling is expected to be a key technology for facilitating the NMR-assignment process and for collecting distance information via spin labeling. To obtain do...