Monoclonal antibodies to the haemagglutinin HA1 subunit of the pandemic influenza A/H1N1 2009 virus and potential application to serodiagnosis

• Published in: Journal of medical virology Vol. 83; no. 4; pp. 559 - 567
• Main Authors: Samuel, Dhanraj, Warrener, Lenesha, Hoschler, Katja
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2011; Wiley; Wiley Subscription Services, Inc; Wiley-Blackwell
• Subjects: Animals; Antibodies, Monoclonal - isolation & purification; Antibodies, Viral - isolation & purification; Biological and medical sciences; Blotting, Western; Ferrets; Fundamental and applied biological sciences. Psychology; HA1 subunit; Hemagglutination Inhibition Tests; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Human viral diseases; Humans; Immunoenzyme Techniques; Infectious diseases; Influenza A Virus, H1N1 Subtype - immunology; influenza A/H1N12009; Medical sciences; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; monoclonal antibodies; Mustela putorius furo; Serologic Tests - methods; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Viral diseases; Virology; Virology - methods; British Isles, England; USA, California; Germany; Australia, Queensland, Brisbane; ISE, Pacific, Solomon Is; Hemagglutinin; Orthomyxoviridae; Monoclonal antibody; Subunit; Infection; Virus; Influenzavirus A; Influenza A virus; Viral disease; influenza A/H1N12009; Influenza A; HA1 subunit; Diagnosis; monoclonal antibodies; Medicine

In order to provide specific serological reagents for pandemic influenza A/H1N1 2009 virus, monoclonal antibodies (Mabs) to recombinant haemagglutinin component HA1 (rHA1) were generated after fusing spleen cells from a mouse immunized with rHA1 protein derived from influenza strain A/California/06/...