Trypsin-like enzyme from sand crab (Portunus pelagicus): purification and characterization

• Published in: Journal of food science Vol. 58; no. 4
• Main Authors: Dionysius, D.A, Hoek, K.S, Milne, J.M, Slattery, S.L
• Format: Journal Article
• Language: English
• Published: 01.07.1993
• Subjects: CANGREJO DE MAR; CRABE; FOIE; HIGADO; PANCREAS; PROTEASAS; PROTEASE; PURIFICACION; PURIFICATION; TRIPSINA; TRYPSINE

Studies with synthetic substrates and specific inhibitors indicated that a proteinase from the hepatopancreas of the sand crab (Portunus pelagicus) was a trypsin like serine proteinase. The molecular weight of the enzyme estimated by gel filtration and mass spectrometry was 25,000, whereas SDS-PAGE...