Is cell culture stressful? Effects of degradable and nondegradable culture surfaces on U937 cell function

• Published in: BioTechniques Vol. 42; no. 6; pp. 744 - 750
• Main Authors: Matheson, Loren A, McBane, Joanne E, Malowany, Janet I, Santerre, J. Paul, Labow, Rosalind S
• Format: Journal Article
• Language: English
• Published: Natick, MA Future Science Ltd 01.06.2007; Eaton; Taylor & Francis Group
• Subjects: Acid Phosphatase - metabolism; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Biodegradation, Environmental; Biological and medical sciences; Carbonates - chemistry; Carbonates - metabolism; Cell Culture Techniques - methods; Cell Physiological Phenomena; Dimethylpolysiloxanes - chemistry; Diverse techniques; Esterases - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Molecular and cellular biology; Polymers - chemistry; Polymers - metabolism; Polystyrenes - chemistry; Proteins - analysis; Proteins - metabolism; Silicones - chemistry; Surface Properties; U937 Cells; Cell culture; Cell surface; Cell function

In vitro cell culture has become one of the most widely used techniques in biological and health sciences research, with the most common culture supports being either tissue culture grade polystyrene (TCPS) or polydimethylsiloxane (PDMS). It has previously been shown that monocyte-derived macrophage...