Phenotypic and Genotypic Evaluation of Aminoglycoside Resistance in Clinical Isolates of Staphylococci in Tehran, Iran
Aminoglycosides play an important role in the treatment of staphylococcal infections, despite the emerging widespread resistance among Staphylococcus . To determine the prevalence of aminoglycoside resistance and aminoglycoside modifying enzyme (AME) genes among infected patients at a teaching hospi...
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Published in | Microbial drug resistance (Larchmont, N.Y.) Vol. 15; no. 2; pp. 129 - 132 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Mary Ann Liebert, Inc
01.06.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Aminoglycosides play an important role in the treatment of staphylococcal infections, despite the emerging widespread resistance among
Staphylococcus
. To determine the prevalence of aminoglycoside resistance and aminoglycoside modifying enzyme (AME) genes among infected patients at a teaching hospital in Tehran, Iran, we tested 585
Staphylococcus
isolates, of which 322 were
Staphylococcus aureus
and 263 were coagulase-negative staphylococci, as determined by the disk diffusion method and multiplex PCR. The minimum inhibitory concentration of gentamicin for each isolate was determined by microbroth dilution. All methicillin-resistant staphylococci were
mecA
-positive by PCR. Of the 585 isolates, 27.6% were susceptible to gentamicin and kanamicin, 27.1% to tobramicin and amikacin, and 21.3% to netilmicin. The most prevalent AME genes included
aac(6′)-Ie-aph(2″)
(93.7%) followed b
y aph(3′)-IIIa
(84.3%) and
ant (4′)-Ia
(28.1%). More than 90% of aminoglycoside-resistant staphylococci contained at least one AME gene. The coexistence of two or three AME genes was detected in most gentamicin-resistant isolates. These results suggest an alarming rate of aminoglycoside resistance in this test location in Tehran, Iran. Continued surveillance at the genotypic and phenotypic levels, and adherence to well-designed antibiotic and infection-control policies are necessary to limit the spread of antimicrobial resistance. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1076-6294 1931-8448 |
DOI: | 10.1089/mdr.2009.0869 |