Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC-MS/MS based protein quantification and metabolic flux analysis

• Published in: Planta Vol. 236; no. 3; pp. 795 - 808
• Main Authors: Wang, Jack P., Shuford, Christopher M., Li, Quanzi, Song, Jina, Lin, Ying-Chung, Sun, Ying-Hsuan, Chen, Hsi-Chuan, Williams, Cranos M., Muddiman, David C., Sederoff, Ronald R., Chiang, Vincent L.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.09.2012; Springer Nature B.V
• Subjects: Agriculture; Alcohols; Biomedical and Life Sciences; Biosynthesis; Cloning, Molecular; Ecology; Enzymatic activity; Enzymes; Flowering plants; Fluctuations; Forestry; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Hydroxylation; Kinetics; Life Sciences; Lignin; Lignin - analysis; Lignin - biosynthesis; Mass spectroscopy; Mixed Function Oxygenases - metabolism; Original Article; Plant Sciences; Plants, Genetically Modified; Populus - metabolism; Protoplasts; Quantification; Xylem; Xylem - enzymology; Yeasts; Yeasts - metabolism; Lignin; Syringyl lignin; Kinetics; Angiosperm; Subcellular localization

Flowering plants have syringyl and guaiacyl subunits in lignin in contrast to the guaiacyl lignin in gymnosperms. The biosynthesis of syringyl subunits is initiated by coniferaldehyde 5-hydroxylase (CAld5H). In Populus trichocarpa there are two closely related CAld5H enzymes (PtrCAld5H1 and PtrCAld5...