Mechanisms Involved in 3′,5′-Cyclic Adenosine Monophosphate-Mediated Inhibition of the Ubiquitin-Proteasome System in Skeletal Muscle

• Published in: Endocrinology (Philadelphia) Vol. 150; no. 12; pp. 5395 - 5404
• Main Authors: Gonçalves, Dawit A. P, Lira, Eduardo C, Baviera, Amanda M, Cao, Peirang, Zanon, Neusa M, Arany, Zoltan, Bedard, Nathalie, Tanksale, Preeti, Wing, Simon S, Lecker, Stewart H, Kettelhut, Isis C, Navegantes, Luiz C. C
• Format: Journal Article
• Language: English
• Published: United States Endocrine Society 01.12.2009; Oxford University Press
• Subjects: 1-Methyl-3-isobutylxanthine - pharmacology; Adenosine monophosphate; Adrenergic beta-2 Receptor Agonists; Agonists; AKT protein; Animals; Atrophy; Biodegradation; Blotting, Western; Catecholamines; Cell Line; Clenbuterol; Clenbuterol - pharmacology; Cyclic AMP; Cyclic AMP - metabolism; Dexamethasone; Dexamethasone - pharmacology; Dietary restrictions; Forkhead Box Protein O3; Forkhead Transcription Factors - metabolism; FOXO3 protein; Gene expression; In Vitro Techniques; Inactivation; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; mRNA; Muscle Proteins - genetics; Muscle Proteins - metabolism; Muscle, Skeletal - cytology; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; Myotubes; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Peroxisome proliferator-activated receptors; Phosphodiesterase inhibitors; Phosphodiesterase Inhibitors - pharmacology; Phosphorylation; Phosphorylation - drug effects; Proteasome Endopeptidase Complex - genetics; Proteasome Endopeptidase Complex - metabolism; Proteasomes; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Rats; Rats, Wistar; Receptors (physiology); Receptors, Adrenergic, beta-2 - genetics; Receptors, Adrenergic, beta-2 - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Skeletal muscle; SKP Cullin F-Box Protein Ligases - genetics; SKP Cullin F-Box Protein Ligases - metabolism; Trans-Activators - genetics; Trans-Activators - metabolism; Transcription Factors; Tripartite Motif Proteins; Ubiquitin - genetics; Ubiquitin - metabolism; Ubiquitin-protein ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism

Although it is well known that catecholamines inhibit skeletal muscle protein degradation, the molecular underlying mechanism remains unclear. This study was undertaken to investigate the role of β2-adrenoceptors (AR) and cAMP in regulating the ubiquitin-proteasome system (UPS) in skeletal muscle. W...