Quantitation of HTLV-I and II proviral load using real-time quantitative PCR with SYBR Green chemistry

• Published in: Journal of clinical virology Vol. 31; no. 4; pp. 275 - 282
• Main Authors: Lee, Tzong-Hae, Chafets, Daniel M., Busch, Michael P., Murphy, Edward L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2004; Elsevier Science
• Subjects: Biological and medical sciences; DNA, Viral - analysis; Fundamental and applied biological sciences. Psychology; HTLV-I and II; HTLV-I Infections - immunology; HTLV-I Infections - virology; HTLV-II Infections - immunology; HTLV-II Infections - virology; Human T-lymphotropic virus 1; Human T-lymphotropic virus 1 - genetics; Human T-lymphotropic virus 1 - physiology; Human T-lymphotropic virus 2; Human T-lymphotropic virus 2 - genetics; Human T-lymphotropic virus 2 - physiology; Human viral diseases; Humans; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Organic Chemicals - metabolism; Polymerase Chain Reaction; Proviral load; Proviruses - genetics; Proviruses - physiology; T cell; Viral diseases; Viral Load; Virology; T cell; HTLV-I and II; Proviral load; Microbiology; Retroviridae; Real time; Provirus; Virology; Virus; Polymerase chain reaction; HTLV-II virus; T-Lymphocyte; HTLV-I virus; Quantitative analysis

Background: Human T-lymphotropic virus type I (HTLV-I) is linked etiologically with adult T cell leukemia/lymphoma and HTLV-I-associated myelopathy/tropical spastic paraparsis (HAM/TSP). Human T-lymphotropic virus type II (HTLV-II) is associated with HAM/TSP and, in HIV coinfected patients only, rar...