Soluble fibrin augments spreading of fibroblasts by providing RGD sequences of fibrinogen in soluble fibrin

• Published in: Thrombosis research Vol. 114; no. 4; pp. 293 - 300
• Main Authors: Yang, Wei, Wu, Bin, Asakura, Shinji, Kohno, Isao, Matsuda, Michio
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Ltd 2004; Elsevier Science
• Subjects: Biological and medical sciences; Blood and lymphatic vessels; Blood vessels and receptors; Cardiology. Vascular system; Cell Adhesion - drug effects; Cell Line; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Disseminated Intravascular Coagulation - blood; Fibrin - isolation & purification; Fibrin - physiology; Fibrinogen - chemistry; Fibrinogen - isolation & purification; Fibroblasts; Fibroblasts - cytology; Fibroblasts - drug effects; Fundamental and applied biological sciences. Psychology; Hormones. Endocrine system; Humans; Medical sciences; Oligopeptides - physiology; Peptide Fragments - chemical synthesis; Peptide Fragments - pharmacology; Pharmacology. Drug treatments; RGD sequences; Solubility; Soluble fibrin; Vertebrates: cardiovascular system; TBS; GPRP; FM; RGD sequences; GRGDSP; Fbg; GRGESP; Soluble fibrin; RGD; mAb; BSA; DMEM; SF; Fibroblasts; Fibrin; Hemostatic; Fibrinogen; Cardiovascular disease; Fibroblast

We previously reported that fibroblasts were found to spread far more avidly on NaBr-solubilized fibrin monomer (FM) monolayers than on immobilized fibrinogen (Fbg), indicating that removal of fibrinopeptides by thrombin is a prerequisite for the fibrin-mediated augmentation of cell spreading [J. Bi...