Transfection of human tumour cells with Mre11 siRNA and the increase in radiation sensitivity and the reduction in heat-induced radiosensitization

• Published in: International journal of hyperthermia Vol. 20; no. 2; pp. 157 - 162
• Main Authors: Xu, M., Myerson, R. J., Hunt, C., Kumar, S., Moros, E. G., Straube, W. L., Roti, J. L. Roti
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.03.2004; Taylor & Francis
• Subjects: Adenocarcinoma; Cell Line, Tumor - cytology; Cell Line, Tumor - physiology; Cell Line, Tumor - radiation effects; Colonic Neoplasms; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene Expression; Gene Silencing; Heat-induced radiosensitization; Humans; Hyperthermia, Induced; Mre11; MRE11 Homologue Protein; Radiation Tolerance - physiology; RNA, Small Interfering; siRNA; Transfection

Double-strand DNA breaks (DSBs) are potentially lethal DNA lesions induced by ionizing radiation. In eukaryotes, DSBs can be repaired by homologous recombination (HR) or non-homologous end-joining (NHEJ). DNA repair protein Mre11 participates in both the NHEJ and HR DNA repair pathways. Hyperthermia...