Crystallographic survey of active sites of an unclassified glutathione transferase from Bombyx mori

• Published in: Biochimica et biophysica acta Vol. 1810; no. 12; pp. 1355 - 1360
• Main Authors: Kakuta, Yoshimitsu, Usuda, Kazuhiro, Nakashima, Takashi, Kimura, Makoto, Aso, Yoichi, Yamamoto, Kohji
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2011
• Subjects: active sites; alanine; Amino Acid Sequence; Animals; Biocatalysis; Bombyx - enzymology; Bombyx mori; catalytic activity; Catalytic Domain; Crystal structure; Crystallography, X-Ray; Glutathione; Glutathione transferase; Glutathione Transferase - chemistry; Glutathione Transferase - metabolism; insecticide resistance; insecticides; Kinetics; Lepidoptera; Models, Molecular; molecular conformation; molecular models; Molecular Sequence Data; Sequence Homology, Amino Acid; silkworms; Site-directed mutagenesis; surveys; SDS-PAGE; Glutathione transferase; Site-directed mutagenesis; Lepidoptera; GST; CDNB; GTX; GSH; Crystal structure; Glutathione

Glutathione transferase (GST) catalyzes a major step in the xenobiotic detoxification pathway. We previously identified a novel, unclassified GST that is upregulated in an insecticide-resistant silkworm ( Bombyx mori) upon insecticide exposure. Here, we sought to further characterize this GST, bmGST...