A rapid, cost-effective method for counting human embryonic stem cell numbers as clumps

• Published in: BioTechniques Vol. 47; no. 1; pp. 599 - 606
• Main Authors: Prowse, Andrew B.J, Wolvetang, Ernst J, Gray, Peter P
• Format: Journal Article
• Language: English
• Published: Natick, MA Future Science Ltd 01.07.2009; Eaton; Taylor & Francis Group
• Subjects: Biological and medical sciences; Biomarkers - analysis; cell count; Cell Count - economics; Cell Culture Techniques - methods; Cell Line; Cell Separation; Coloring Agents - metabolism; Detergents - pharmacology; Diverse techniques; DNA - metabolism; Embryonic Stem Cells - cytology; Endopeptidases - pharmacology; fluorescence; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; high throughput; human embryonic stem cells; Humans; Molecular and cellular biology; Octoxynol - pharmacology; Propidium - metabolism; propidium iodide; Reproducibility of Results; Time Factors; Trypsin - pharmacology; Human; Costs; Method; Embryonic cell; Stem cell; Counting

Enumeration of human embryonic stem cell (hESC) numbers through single cell digestion can be time consuming especially in high-throughput or multi-factorial analysis containing 50+ samples. We have developed a reproducible, cost-effective method of counting hESCs in clumps circumventing the need to...