Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo

• Published in: Toxicological research (Seoul) Vol. 34; no. 2; pp. 133 - 141
• Main Authors: Nho, Jong-Hyun, Jung, Ho-Kyung, Lee, Mu-Jin, Jang, Ji-Hun, Sim, Mi-Ok, Jeong, Da-Eun, Cho, Hyun-Woo, Kim, Jong-Choon
• Format: Journal Article
• Language: English
• Published: Singapore 한국독성학회 01.04.2018; Springer Singapore; Korean Society of Toxicology
• Subjects: Biomedicine; Original; Original Article; Pharmacology/Toxicology; 예방의학; HK-2; Cynaroside; Nephrotoxicity; MST-1; Cisplatin
• ISSN: 1976-8257; 2234-2753
• DOI: 10.5487/TR.2018.34.2.133

Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that 10 µM cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo , we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo , and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/ MST-1-linked signal transduction in the animal model.