Release of calcium from suspension-cultured Glycine max cells by chitosan, other polycations, and polyamines in relation to effects on membrane permeability [Soybean]

Treatment with chitosan of suspension-cultured Glycine max cells labeled with ^{45}\text{Ca}^{2+}$ caused a rapid release of calcium, which was complete much earlier than the chitosan-induced leakage of intracellular electrolytes and probably reflects calcium loss primarily from the cell wall and/or...

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Published inPlant physiology (Bethesda) Vol. 73; no. 3; pp. 698 - 702
Main Authors Young, David H., Kauss, Heinrich
Format Journal Article
LanguageEnglish
Published Rockville, MD American Society of Plant Physiologists 01.11.1983
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Abstract Treatment with chitosan of suspension-cultured Glycine max cells labeled with ^{45}\text{Ca}^{2+}$ caused a rapid release of calcium, which was complete much earlier than the chitosan-induced leakage of intracellular electrolytes and probably reflects calcium loss primarily from the cell wall and/or plasma membrane. A linear correlation was found between calcium release from chitosan-treated whole cells or isolated cell walls and the amount of chitosan bound. Other polycations (poly-L-lysine, histone, DEAE-dextran, and protamine sulfate), low molecular weight polyamines (spermine, spermidine, and putrescine) and polyanions (polygalacturonate and poly-L-aspartate, which act as chelating agents) also released calcium from whole cells and isolated cell walls; however, only the polycations increased membrane permeability. Poly-L-lysines of differing molecular weight showed a similar ability to release calcium, but their effect on membrane permeability increased with increasing molecular weight. The results suggest that the effect of polycations on permeability is not the direct result of calcium displacement from the cell surface but is probably due to cross-linking of surface components. The order of effectiveness of inorganic cations in displacing calcium from whole cells and isolated cell walls was Ca2+, Ba2+, Sr2+ > Mg2+ > K+, Na+.
AbstractList Treatment with chitosan of suspension-cultured Glycine max cells labeled with (45)Ca(2+) caused a rapid release of calcium, which was complete much earlier than the chitosan-induced leakage of intracellular electrolytes and probably reflects calcium loss primarily from the cell wall and/or plasma membrane. A linear correlation was found between calcium release from chitosan-treated whole cells or isolated cell walls and the amount of chitosan bound. Other polycations (poly-l-lysine, histone, DEAE-dextran, and protamine sulfate), low molecular weight polyamines (spermine, spermidine, and putrescine) and polyanions (polygalacturonate and poly-l-aspartate, which act as chelating agents) also released calcium from whole cells and isolated cell walls; however, only the polycations increased membrane permeability. Poly-l-lysines of differing molecular weight showed a similar ability to release calcium, but their effect on membrane permeability increased with increasing molecular weight. The results suggest that the effect of polycations on permeability is not the direct result of calcium displacement from the cell surface but is probably due to cross-linking of surface components. The order of effectiveness of inorganic cations in displacing calcium from whole cells and isolated cell walls was Ca(2+), Ba(2+), Sr(2+) > Mg(2+) > K(+), Na(+).
Treatment with chitosan of suspension-cultured Glycine max cells labeled with super(45)Ca super(2+) caused a rapid release of calcium, which was complete much earlier than the chitosan-induced leakage of intracellular electrolytes and probably reflects calcium loss primarily from the cell wall and/or plasma membrane. A linear correlation was found between calcium release from chitosan-treated whole cells or isolated cell walls and the amount of chitosan bound. Other polycations (Poly-L-lysine, histone, DEAE-dextran, and protamine sulfate), low molecular weight polyamines (spermine, spermidine, and putrescine) and polyanions (polygalacturonate and poly-L-aspartate, which act as chelating agents) also released calcium from whole cells and isolated cell walls; however, only the polycations increased membrane permeability. The results suggest that the effect of polycations on permeability is not the direct result of calcium displacement from the cell surface but is probably due to cross-linking of surface components.
Treatment with chitosan of suspension-cultured Glycine max cells labeled with ^{45}\text{Ca}^{2+}$ caused a rapid release of calcium, which was complete much earlier than the chitosan-induced leakage of intracellular electrolytes and probably reflects calcium loss primarily from the cell wall and/or plasma membrane. A linear correlation was found between calcium release from chitosan-treated whole cells or isolated cell walls and the amount of chitosan bound. Other polycations (poly-L-lysine, histone, DEAE-dextran, and protamine sulfate), low molecular weight polyamines (spermine, spermidine, and putrescine) and polyanions (polygalacturonate and poly-L-aspartate, which act as chelating agents) also released calcium from whole cells and isolated cell walls; however, only the polycations increased membrane permeability. Poly-L-lysines of differing molecular weight showed a similar ability to release calcium, but their effect on membrane permeability increased with increasing molecular weight. The results suggest that the effect of polycations on permeability is not the direct result of calcium displacement from the cell surface but is probably due to cross-linking of surface components. The order of effectiveness of inorganic cations in displacing calcium from whole cells and isolated cell walls was Ca2+, Ba2+, Sr2+ > Mg2+ > K+, Na+.
Author Young D.H
Kauss H
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Issue 3
Keywords Leguminosae
Calcium
Cell permeability
Dicotyledones
Angiospermae
Tissu culture
Spermatophyta
Chitosan
Fodder crop
Inorganic element
Glycine max
Oil plant(vegetal)
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Snippet Treatment with chitosan of suspension-cultured Glycine max cells labeled with ^{45}\text{Ca}^{2+}$ caused a rapid release of calcium, which was complete much...
Treatment with chitosan of suspension-cultured Glycine max cells labeled with (45)Ca(2+) caused a rapid release of calcium, which was complete much earlier...
Treatment with chitosan of suspension-cultured Glycine max cells labeled with super(45)Ca super(2+) caused a rapid release of calcium, which was complete much...
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SubjectTerms Agronomy. Soil science and plant productions
Biological and medical sciences
Calcium
Cell membranes
Cell separation
Cell walls
Chemical suspensions
chitosan
Electrolytes
Electrolytic cells
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Glycine max
membrane permeability
Plant physiology and development
Plants
Polyamines
polycations
Polymers
Tissue cultures, protoplasts
Vegetative propagation. Micropropagation
Vegetative propagation. Sowing and planting. Harvesting
Title Release of calcium from suspension-cultured Glycine max cells by chitosan, other polycations, and polyamines in relation to effects on membrane permeability [Soybean]
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