Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons

• Published in: Bio-protocol Vol. 13; no. 12; p. e4697
• Main Authors: Mangalath, Aswathy, Raj, Vishnu, Santhosh, Rasitha, Thekkuveettil, Anoopkumar
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 20.06.2023; Bio-protocol LLC
• Subjects: Biology; Methods; Calcium imaging; Immobilization; C. elegans; Sodium alginate; Live recording; Microfluidic

A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in is challenging due to the difficulties associated with imm...