Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons
A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in is challenging due to the difficulties associated with imm...
Saved in:
| Published in | Bio-protocol Vol. 13; no. 12; p. e4697 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Bio-Protocol
20.06.2023
Bio-protocol LLC |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons.
is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in
is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation. |
|---|---|
| AbstractList | A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons.
is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in
is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation. A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation. A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation. A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation.A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation. |
| Author | Thekkuveettil, Anoopkumar Santhosh, Rasitha Raj, Vishnu Mangalath, Aswathy |
| Author_xml | – sequence: 1 givenname: Aswathy surname: Mangalath fullname: Mangalath, Aswathy – sequence: 2 givenname: Vishnu surname: Raj fullname: Raj, Vishnu – sequence: 3 givenname: Rasitha surname: Santhosh fullname: Santhosh, Rasitha – sequence: 4 givenname: Anoopkumar surname: Thekkuveettil fullname: Thekkuveettil, Anoopkumar |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37397799$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1ks9LHDEUx0OxVKueeytz7GU1vzaTORW7tHZBag8ehZBkXsZIJtkmM4L-9Y27KlooOSS8fL-f74P3PqK9mCIg9IngE0pa0Z1-8-l3TlOyJ1x07Tt0QBkjC8nocu_Vex8dl3KLMSZMUCL4B7TPWta1bdcdoOuzMPioJ2jOITTrcUzGB_-gJ59ik1yz0hBTvtGm95MvDQQYdCyNS7m53Eze6lAlwfp5rGZdUcOj6xfMOcVyhN47HQocP92H6OrH96vVz8XF5fl6dXaxsFzgaSE17QGosAxLEI4BI9ZYV4_gknDSW2wYoQa73vXSyI4byyyrBS2kdOwQrXfYPulbtcl-1PleJe3VtpDyoHSuvQZQS4G1tMwQ2grOGe96pzvZOWnM0vSCVtbXHWszmxF6C3HKOryBvv2J_kYN6U4RXLsnsq2EL0-EnP7MUCY1-mIhBB0hzUXROhPJGRWySj-_DntJeR5PFSx3AptTKRmcsn7azqZm-1BD1XYT1MsmqMdNqL7Tf3zP6P85_gJBBrpp |
| CitedBy_id | crossref_primary_10_1016_j_bbrc_2023_07_002 crossref_primary_10_1371_journal_pone_0300628 crossref_primary_10_3390_ijms25031861 |
| Cites_doi | 10.1016/s0896-6273(00)81196-4 10.1371/journal.pone.0193989 10.1016/b978-0-12-394620-1.00006-0 10.1039/b707861g 10.1038/nmeth.2019 10.1371/journal.pone.0053419 10.1093/genetics/95.4.905 10.1002/jnr.25112/v3/review1 |
| ContentType | Journal Article |
| Copyright | Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license. Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license 2023 |
| Copyright_xml | – notice: Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license. – notice: Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license 2023 |
| DBID | AAYXX CITATION NPM 7X8 5PM DOA |
| DOI | 10.21769/BioProtoc.4697 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2331-8325 |
| ExternalDocumentID | oai_doaj_org_article_560a8c3b127644349dfa989f8bb5bd62 PMC10308187 37397799 10_21769_BioProtoc_4697 |
| Genre | Journal Article |
| GroupedDBID | 53G AAYXX ALMA_UNASSIGNED_HOLDINGS CITATION FRP GROUPED_DOAJ M~E OK1 RPM NPM 7X8 5PM |
| ID | FETCH-LOGICAL-c460t-8a2dee26c308e6f3e31cbcfcfc648141dc0b312b0fdfd8b894bc3c32b0a688f3 |
| IEDL.DBID | DOA |
| ISSN | 2331-8325 |
| IngestDate | Wed Aug 27 01:21:14 EDT 2025 Thu Aug 21 18:37:42 EDT 2025 Fri Jul 11 07:26:33 EDT 2025 Thu Jan 02 22:51:42 EST 2025 Tue Jul 01 03:41:47 EDT 2025 Thu Apr 24 23:01:17 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 12 |
| Keywords | Calcium imaging Immobilization C. elegans Sodium alginate Live recording Microfluidic |
| Language | English |
| License | Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license. This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c460t-8a2dee26c308e6f3e31cbcfcfc648141dc0b312b0fdfd8b894bc3c32b0a688f3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://doaj.org/article/560a8c3b127644349dfa989f8bb5bd62 |
| PMID | 37397799 |
| PQID | 2832843268 |
| PQPubID | 23479 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_560a8c3b127644349dfa989f8bb5bd62 pubmedcentral_primary_oai_pubmedcentral_nih_gov_10308187 proquest_miscellaneous_2832843268 pubmed_primary_37397799 crossref_citationtrail_10_21769_BioProtoc_4697 crossref_primary_10_21769_BioProtoc_4697 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2023-06-20 |
| PublicationDateYYYYMMDD | 2023-06-20 |
| PublicationDate_xml | – month: 06 year: 2023 text: 2023-06-20 day: 20 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: 1075 Lorne Way, Sunnyvale, CA 94087, USA |
| PublicationTitle | Bio-protocol |
| PublicationTitleAlternate | Bio Protoc |
| PublicationYear | 2023 |
| Publisher | Bio-Protocol Bio-protocol LLC |
| Publisher_xml | – name: Bio-Protocol – name: Bio-protocol LLC |
| References | ref246269 ref246265 ref246266 ref246267 ref246268 ref246272 ref246270 ref246271 |
| References_xml | – ident: ref246268 doi: 10.1016/s0896-6273(00)81196-4 – ident: ref246265 doi: 10.1371/journal.pone.0193989 – ident: ref246266 doi: 10.1016/b978-0-12-394620-1.00006-0 – ident: ref246267 doi: 10.1039/b707861g – ident: ref246272 doi: 10.1038/nmeth.2019 – ident: ref246269 doi: 10.1371/journal.pone.0053419 – ident: ref246270 doi: 10.1093/genetics/95.4.905 – ident: ref246271 doi: 10.1002/jnr.25112/v3/review1 |
| SSID | ssj0001362164 |
| Score | 2.2518568 |
| Snippet | A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons.
is one of the most suitable models for optically recording... A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for... A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for... |
| SourceID | doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e4697 |
| SubjectTerms | Biology Methods |
| Title | Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/37397799 https://www.proquest.com/docview/2832843268 https://pubmed.ncbi.nlm.nih.gov/PMC10308187 https://doaj.org/article/560a8c3b127644349dfa989f8bb5bd62 |
| Volume | 13 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQT70gEBQCBblSD72kTWLHsY9txXZBonAo0h6QIj_G2pWWpNpm_z8zzna1W4G4IN_8UKzxxPPI5PsYOw1NVLbwkAtVQi6jc7mrbJOXEGzj6qhLS3nIr7dq-kN-mdWzHaovqgkb4YFHwV2gRbbaC1dWDZpuIU2I1mgTtXO1C-PtizZvJ5hK2RW8lzEQGLF80OtW5uJq0X9f9UPvzzEibPbMUELr_5OL-bRScsf0TF6w5xufkV-Oe33JnkH3iv28XBKlwgD8Bpb8M6oTlbmOP1XyPvJrC12_mlsXCLWIE70EWiWOPir_dp8S2Dhl6RfrX7g4URXRqoTV0T28ZneTT3fX03xDlZB7qYoh17YKAJXyotCgImU2vfMRm5K6lGXwhRNl5YoYYtBOG-m88AI7rNI6iiN20PUdvGUc4xsAiBZbLXHY1CjxIpgavDDCyoydPwqu9RsYcWKzWLYYTiRJt1tJtyTpjJ1tF9yPCBp_n3pFJ7GdRtDXqQMVot0oRPsvhcjYyeM5tviq0PcP20G_fmiJlUlL9Fd1xt6M57p9lGjIEzYmY3rvxPf2sj_SLeYJjpuI2tDtad79j92_Z4dEaE_FaFVxzA6G1Ro-oNszuI9Jw38DZxkGrw |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Alginate+Gel+Immobilization+of+Caenorhabditis+elegans+for+Optical+Calcium+Imaging+of+Neurons&rft.jtitle=Bio-protocol&rft.au=Mangalath%2C+Aswathy&rft.au=Raj%2C+Vishnu&rft.au=Santhosh%2C+Rasitha&rft.au=Thekkuveettil%2C+Anoopkumar&rft.date=2023-06-20&rft.eissn=2331-8325&rft.volume=13&rft.issue=12&rft.spage=e4697&rft_id=info:doi/10.21769%2FBioProtoc.4697&rft_id=info%3Apmid%2F37397799&rft.externalDocID=37397799 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2331-8325&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2331-8325&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2331-8325&client=summon |