Rapid extraction and analysis of oxidative stress and DNA damage biomarker 8-hydroxy-2′-deoxyguanosine (8-OHdG) in urine: Application to a study with pregnant women

• Published in: International journal of hygiene and environmental health Vol. 250; p. 114175
• Main Authors: Bláhová, Lucie, Janoš, Tomáš, Mustieles, Vicente, Rodríguez-Carrillo, Andrea, Fernández, Mariana F., Bláha, Luděk
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.05.2023; Urban & Fischer
• Subjects: 8-Hydroxy-2'-Deoxyguanosine; 8-OHdG; Biomarkers - urine; Chromatography, Liquid - methods; Creatinine - urine; Deoxyguanosine - urine; DNA Damage; Female; Humans; Infant, Newborn; Male; Oxidative Stress; Placenta; Pregnancy; Pregnant Women; Tandem Mass Spectrometry - methods; Urine; Urine; Pregnancy; Oxidative stress; Placenta; 8-OHdG

Oxidative stress is an important toxicity and genotoxicity mechanism of many chronic adverse health outcomes. This study developed a sensitive extraction method for urine matrix (based on lyophilization, without the need for pre-cleaning by solid phase extraction), coupled to LC-MS/MS analysis of th...