Neuroprotective and anti-inflammatory effects of eicosane on glutamate and NMDA-induced retinal ganglion cell injury
AIM: To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-D-aspartate (NMDA)-induced retinal ganglion cell (RGC) injury in a mouse model of glaucoma. METHODS: The protective effects of eicosane on...
Saved in:
| Published in | International journal of ophthalmology Vol. 17; no. 4; pp. 638 - 645 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
China
International Journal of Ophthalmology Press
18.04.2024
Press of International Journal of Ophthalmology (IJO PRESS) |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | AIM: To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-D-aspartate (NMDA)-induced retinal ganglion cell (RGC) injury in a mouse model of glaucoma.
METHODS: The protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide staining. Intracellular reactive oxygen species (ROS) production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow cytometry. The protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of retina. The effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatography-mass spectroscopy (LC-MS) and untargeted metabolomics analyses.
RESULTS: Eicosane treatment significantly attenuated glutamate-induced damage to R28 cells in vitro. Eicosane also protected RGCs against NMDA-induced injury in a mouse glaucoma model. Untargeted metabolomics analyses showed that eicosane increased multiple metabolites, including L-arginine and L-carnitine, in the retina.
CONCLUSION: Eicosane has protective effects, antioxidant potential, and anti-inflammatory properties in an in vitro model of glutamate-induced cell damage and in an in vivo model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism. |
|---|---|
| AbstractList | AIM: To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-D-aspartate (NMDA)-induced retinal ganglion cell (RGC) injury in a mouse model of glaucoma. METHODS: The protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide staining. Intracellular reactive oxygen species (ROS) production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow cytometry. The protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of retina. The effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatography-mass spectroscopy (LC-MS) and untargeted metabolomics analyses. RESULTS: Eicosane treatment significantly attenuated glutamate-induced damage to R28 cells in vitro. Eicosane also protected RGCs against NMDA-induced injury in a mouse glaucoma model. Untargeted metabolomics analyses showed that eicosane increased multiple metabolites, including L-arginine and L-carnitine, in the retina. CONCLUSION: Eicosane has protective effects, antioxidant potential, and anti-inflammatory properties in an in vitro model of glutamate-induced cell damage and in an in vivo model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism. AIM: To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-D-aspartate (NMDA)-induced retinal ganglion cell (RGC) injury in a mouse model of glaucoma. METHODS: The protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide staining. Intracellular reactive oxygen species (ROS) production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow cytometry. The protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of retina. The effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatography-mass spectroscopy (LC-MS) and untargeted metabolomics analyses. RESULTS: Eicosane treatment significantly attenuated glutamate-induced damage to R28 cells in vitro. Eicosane also protected RGCs against NMDA-induced injury in a mouse glaucoma model. Untargeted metabolomics analyses showed that eicosane increased multiple metabolites, including L-arginine and L-carnitine, in the retina. CONCLUSION: Eicosane has protective effects, antioxidant potential, and anti-inflammatory properties in an in vitro model of glutamate-induced cell damage and in an in vivo model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism. To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-D-aspartate (NMDA)-induced retinal ganglion cell (RGC) injury in a mouse model of glaucoma.AIMTo investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-D-aspartate (NMDA)-induced retinal ganglion cell (RGC) injury in a mouse model of glaucoma.The protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide staining. Intracellular reactive oxygen species (ROS) production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow cytometry. The protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of retina. The effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatography-mass spectroscopy (LC-MS) and untargeted metabolomics analyses.METHODSThe protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide staining. Intracellular reactive oxygen species (ROS) production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow cytometry. The protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of retina. The effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatography-mass spectroscopy (LC-MS) and untargeted metabolomics analyses.Eicosane treatment significantly attenuated glutamate-induced damage to R28 cells in vitro. Eicosane also protected RGCs against NMDA-induced injury in a mouse glaucoma model. Untargeted metabolomics analyses showed that eicosane increased multiple metabolites, including L-arginine and L-carnitine, in the retina.RESULTSEicosane treatment significantly attenuated glutamate-induced damage to R28 cells in vitro. Eicosane also protected RGCs against NMDA-induced injury in a mouse glaucoma model. Untargeted metabolomics analyses showed that eicosane increased multiple metabolites, including L-arginine and L-carnitine, in the retina.Eicosane has protective effects, antioxidant potential, and anti-inflammatory properties in an in vitro model of glutamate-induced cell damage and in an in vivo model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism.CONCLUSIONEicosane has protective effects, antioxidant potential, and anti-inflammatory properties in an in vitro model of glutamate-induced cell damage and in an in vivo model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism. To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-D-aspartate (NMDA)-induced retinal ganglion cell (RGC) injury in a mouse model of glaucoma. The protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide staining. Intracellular reactive oxygen species (ROS) production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow cytometry. The protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of retina. The effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatography-mass spectroscopy (LC-MS) and untargeted metabolomics analyses. Eicosane treatment significantly attenuated glutamate-induced damage to R28 cells . Eicosane also protected RGCs against NMDA-induced injury in a mouse glaucoma model. Untargeted metabolomics analyses showed that eicosane increased multiple metabolites, including L-arginine and L-carnitine, in the retina. Eicosane has protective effects, antioxidant potential, and anti-inflammatory properties in an model of glutamate-induced cell damage and in an model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism. |
| Author | Zhong, Jing-Xiang Zhu, You-Lin Xiong, Meng-Qin Wu, Zhen-Kai |
| AuthorAffiliation | 1 Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China 2 Changde Hospital, Xiangya School of Medicine, Central South University (the First People's Hospital of Changde City), Changde 415000, Hunan Province, China |
| AuthorAffiliation_xml | – name: 1 Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China – name: 2 Changde Hospital, Xiangya School of Medicine, Central South University (the First People's Hospital of Changde City), Changde 415000, Hunan Province, China |
| Author_xml | – sequence: 1 givenname: Zhen-Kai surname: Wu fullname: Wu, Zhen-Kai – sequence: 2 givenname: You-Lin surname: Zhu fullname: Zhu, You-Lin – sequence: 3 givenname: Meng-Qin surname: Xiong fullname: Xiong, Meng-Qin – sequence: 4 givenname: Jing-Xiang surname: Zhong fullname: Zhong, Jing-Xiang |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38638263$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1Uktv1DAQtlARfdAzN5Qjl2z9jn1CVQu0UikXOFsTxw5eZe3FcSrtv8fdbRFFwhprLM_3sMZzio5iig6hdwSviKIcX4R1WlFM-QrXEK_QCaW0a7nS6mh_pi3TQh-j83le47qkwATzN-iYKckUlewElXu35LTNqThbwoNrIA51l9CG6CfYbKCkvGuc97U-N8k3Ltg0Q3RNis04LQUq5EC7_3p9WWnDYt3QZFdChKkZIY5TqFjrpqkJcb3k3Vv02sM0u_OnfIZ-fP70_eqmvfv25fbq8q61XOLSSsE09QORVHQcdO96TZTTAIxy0Xk8UNBgvfBWy05yrCzpRG-9AgVgHWdn6PagOyRYm20OG8g7kyCY_UXKo4Fcgp2cwZJS3QsFAgjHQvRdNcPE1id0vWW-an08aG2XfuMG62LJML0QfVmJ4acZ04MhWCuFZVcVPjwp5PRrcXMxmzA_dqU2My2zYZgz3BGtZYW-_9vsj8vzv1WAOABsTvOcnTc2FCi1z9U7TNXU7EfE1BExjyNicA1ReRf_8J6l_8f4DbhmwAs |
| CitedBy_id | crossref_primary_10_3390_microorganisms13040716 crossref_primary_10_1016_j_mtbio_2024_101229 crossref_primary_10_1016_j_sajb_2025_02_012 |
| ContentType | Journal Article |
| Copyright | International Journal of Ophthalmology Press. International Journal of Ophthalmology Press 2024 |
| Copyright_xml | – notice: International Journal of Ophthalmology Press. – notice: International Journal of Ophthalmology Press 2024 |
| CorporateAuthor | Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China; Changde Hospital, Xiangya School of Medicine, Central South University (the First People's Hospital of Changde City), Changde 415000, Hunan Province, China Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China Changde Hospital, Xiangya School of Medicine, Central South University (the First People's Hospital of Changde City), Changde 415000, Hunan Province, China |
| CorporateAuthor_xml | – name: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China; Changde Hospital, Xiangya School of Medicine, Central South University (the First People's Hospital of Changde City), Changde 415000, Hunan Province, China – name: Changde Hospital, Xiangya School of Medicine, Central South University (the First People's Hospital of Changde City), Changde 415000, Hunan Province, China – name: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China |
| DBID | AAYXX CITATION NPM 7X8 5PM DOA |
| DOI | 10.18240/ijo.2024.04.05 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE - Academic PubMed |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 2227-4898 |
| EndPage | 645 |
| ExternalDocumentID | oai_doaj_org_article_06229b58a5a14055b7b9101c5397bc3f PMC10988067 38638263 10_18240_ijo_2024_04_05 |
| Genre | Journal Article |
| GroupedDBID | 53G 5VR 5VS AAYXX ABKZE AENEX ALMA_UNASSIGNED_HOLDINGS CCEZO CHBEP CIEJG CITATION DIK FA0 GROUPED_DOAJ HYE RNS RPM AFUIB IPNFZ NPM RIG 7X8 5PM |
| ID | FETCH-LOGICAL-c460t-65392fd162574a9beb918e9aa32457f0d2a9acf5fc9676408c175bcf8a8aace43 |
| IEDL.DBID | DOA |
| ISSN | 2222-3959 |
| IngestDate | Wed Aug 27 01:31:41 EDT 2025 Thu Aug 21 18:34:08 EDT 2025 Thu Jul 10 22:49:13 EDT 2025 Thu Apr 03 07:00:06 EDT 2025 Tue Jul 01 04:15:16 EDT 2025 Thu Apr 24 23:05:30 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | metabolites glaucoma eicosane |
| Language | English |
| License | International Journal of Ophthalmology Press. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c460t-65392fd162574a9beb918e9aa32457f0d2a9acf5fc9676408c175bcf8a8aace43 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://doaj.org/article/06229b58a5a14055b7b9101c5397bc3f |
| PMID | 38638263 |
| PQID | 3043071996 |
| PQPubID | 23479 |
| PageCount | 8 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_06229b58a5a14055b7b9101c5397bc3f pubmedcentral_primary_oai_pubmedcentral_nih_gov_10988067 proquest_miscellaneous_3043071996 pubmed_primary_38638263 crossref_citationtrail_10_18240_ijo_2024_04_05 crossref_primary_10_18240_ijo_2024_04_05 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2024-04-18 |
| PublicationDateYYYYMMDD | 2024-04-18 |
| PublicationDate_xml | – month: 04 year: 2024 text: 2024-04-18 day: 18 |
| PublicationDecade | 2020 |
| PublicationPlace | China |
| PublicationPlace_xml | – name: China |
| PublicationTitle | International journal of ophthalmology |
| PublicationTitleAlternate | Int J Ophthalmol |
| PublicationYear | 2024 |
| Publisher | International Journal of Ophthalmology Press Press of International Journal of Ophthalmology (IJO PRESS) |
| Publisher_xml | – name: International Journal of Ophthalmology Press – name: Press of International Journal of Ophthalmology (IJO PRESS) |
| SSID | ssj0000650104 |
| Score | 2.3270066 |
| Snippet | AIM: To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on... To investigate the protective effects, antioxidant potential, and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on... |
| SourceID | doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 638 |
| SubjectTerms | Basic Research eicosane glaucoma metabolites |
| Title | Neuroprotective and anti-inflammatory effects of eicosane on glutamate and NMDA-induced retinal ganglion cell injury |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/38638263 https://www.proquest.com/docview/3043071996 https://pubmed.ncbi.nlm.nih.gov/PMC10988067 https://doaj.org/article/06229b58a5a14055b7b9101c5397bc3f |
| Volume | 17 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELaqHlAviJYCoS0yEgcuoYnj57EFqgqpPbVSb9b4VbaCLKLp_2cmSVe7CMQFKbkktmLNN55HPP7M2DsIqiQVZd2CCbUkNQZnck1MMdoEjV5mLJC91OfX8suNulk76otqwiZ64Elwx40WwgVlQQHmAkoFE9DDtVGhIw2xK2R90eetJVOTDVaUaNDJcoLK1Z1yM6-PRRd2vLijfX9CjiynasMljcz9fwo3f6-aXHNDZ8_Y0zl-5CfTuHfZVu732JOLeYX8ORtGto2ZfQEtGYc-4T0salQlRP_7uKrO5zIOviw8oy7cQ5_5sue3qIeATaZulxefTrBbQvQTp92O9OVboI2_2JZ--fNFf4eg7LPrs89XH8_r-WSFOkrdDDXx0YqSWkx-jAQXMsrUZgeA4ZUypUkCHMSiSnTaaNnYiFFGiMWCBYhZdi_Ydr_s8yvGdUhtCDoVm4p0QgSnQggNQMZkMrpcsQ-PwvVxph2n0y--eUo_CA2PaHhCwzd4qYq9X3X4MTFu_L3pKaG1akZU2eMDVCA_K5D_lwJV7O0j1h6nFgkPZb58uPcd8aEZKtOu2MsJ-9WnOouGS-iuYnZDKzbGsvmmX3wd6bvbxqHR1Ob1_xj9AdshedD6VmsP2fbw8yEfYZg0hDfjjPgFs2gPZA |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Neuroprotective+and+anti-inflammatory+effects+of+eicosane+on+glutamate+and+NMDA-induced+retinal+ganglion+cell+injury&rft.jtitle=International+journal+of+ophthalmology&rft.au=Wu%2C+Zhen-Kai&rft.au=Li%2C+Huan-Yu&rft.au=Zhu%2C+You-Lin&rft.au=Xiong%2C+Meng-Qin&rft.date=2024-04-18&rft.issn=2222-3959&rft.volume=17&rft.issue=4&rft.spage=638&rft_id=info:doi/10.18240%2Fijo.2024.04.05&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2222-3959&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2222-3959&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2222-3959&client=summon |