Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves

• Published in: Journal of Integrative Agriculture Vol. 16; no. 4; pp. 884 - 891
• Main Authors: LIU, Zhen, SUNZHU, Yuan-ji, ZHOU, Xue-ping, HONG, Jian, WU, Jian-xiang
• Format: Journal Article
• Language: English
• Published: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China 01.04.2017; KeAi Communications Co., Ltd
• Subjects: antigens; ascites; China; Citrus; Citrus yellow vein clearing virus; coat proteins; Dot-ELISA; enzyme-linked immunosorbent assay; leaves; Mandarivirus; mice; monoclonal antibodies; monoclonal antibody; orchards; polyclonal antibodies; serological assay; viruses; 单克隆抗体; 斑点酶联免疫吸附试验; 柑桔园; 柑橘园; 清除病毒; 血清学检测; 酶联免疫吸附测定; China; monoclonal antibody; serological assay; Citrus yellow vein clearing virus

Citrus yellow vein clearing virus （CYVCV） is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the family Alphaflexiviridae. Capsid protein （CP） of CYVCV Chongqing isolate （CYVCV- CQ） was produced using a prokaryotic expression system and...