Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves
Citrus yellow vein clearing virus （CYVCV） is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the family Alphaflexiviridae. Capsid protein （CP） of CYVCV Chongqing isolate （CYVCV- CQ） was produced using a prokaryotic expression system and...
Saved in:
| Published in | Journal of Integrative Agriculture Vol. 16; no. 4; pp. 884 - 891 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
01.04.2017
KeAi Communications Co., Ltd |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Citrus yellow vein clearing virus （CYVCV） is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the family Alphaflexiviridae. Capsid protein （CP） of CYVCV Chongqing isolate （CYVCV- CQ） was produced using a prokaryotic expression system and used as the immunogen for monoclonal antibody （MAb） production. Four highly specific and sensitive murine MAbs and one polyclonal antibody were prepared in this study. Titers of the four MAbs in ascites fluids ranged from 10-6 to 10-7 as determined by indirect enzyme-linked immunosorbent assay （ELISA）. Three serological assays, including dot enzyme-linked immunosorbent assay （dot-ELISA）, tissue blot-ELISA, and double-antibody sandwich （DAS）-ELISA, were developed for quick and reliable detections of CYVCV in citrus samples. The developed dot-ELISA and DAS-ELISA methods could detect CYVCV in the infected citrus leaf crude extracts diluted at 1：2 560 and 1：10 240 （w/v, g mL^-1）, respectively. The detection result of 125 citrus leaf samples collected from citrus groves in Yunnan Province and Chongqing Municipality of China showed that approximately 36% samples were positive for CYVCV. This virus was, however, not＇detected in any sample collected from Zhejiang or Jiangxi Province, China. |
|---|---|
| AbstractList | Citrus yellow vein clearing virus (CYVCV) is considered as the causal agent of Citrus yellow vein clearing disease and be-longs to the genus Mandarivirus in the family Alphaflexiviridae. Capsid protein (CP) of CYVCV Chongqing isolate (CYVCV-CQ) was produced using a prokaryotic expression system and used as the immunogen for monoclonal antibody (MAb) production. Four highly specific and sensitive murine MAbs and one polyclonal antibody were prepared in this study. Titers of the four MAbs in ascites fluids ranged from 10−6 to 10−7 as determined by indirect enzyme-linked immunosorbent assay (ELISA). Three serological assays, including dot enzyme-linked immunosorbent assay (dot-ELISA), tissue blot-ELISA, and double-antibody sandwich (DAS)-ELISA, were developed for quick and reliable detections of CYVCV in citrus samples. The developed dot-ELISA and DAS-ELISA methods could detect CYVCV in the infected citrus leaf crude extracts diluted at 1:2 560 and 1:10 240 (w/v, g mL−1), respectively. The detection result of 125 citrus leaf samples collected from citrus groves in Yunnan Province and Chongqing Municipality of China showed that approximately 36% samples were positive for CYVCV. This virus was, however, not detected in any sample collected from Zhejiang or Jiangxi Province, China. Citrus yellow vein clearing virus (CYVCV) is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the family Alphaflexiviridae.Capsid protein (CP) of CYVCV Chongqing isolate (CYVCVCQ) was produced using a prokaryotic expression system and used as the immunogen for monoclonal antibody (MAb) production.Four highly specific and sensitive murine MAbs and one polyclonal antibody were prepared in this study.Titers of the four MAbs in ascites fluids ranged from 10-6 to 10-7 as determined by indirect enzyme-linked immunosorbent assay (ELISA).Three serological assays,including dot enzyme-linked immunosorbent assay (dot-ELISA),tissue blot-ELISA,and double-antibody sandwich (DAS)-ELISA,were developed for quick and reliable detections of CYVCV in citrus samples.The developed dot-ELISA and DAS-ELISA methods could detect CYVCV in the infected citrus leaf crude extracts diluted at 1:2560 and 1:10240 (w/v,g mL-1),respectively.The detection result of 125 citrus leaf samples collected from citrus groves in Yunnan Province and Chongqing Municipality of China showed that approximately 36％ samples were positive for CYVCV.This virus was,however,not detected in any sample collected from Zhejiang or Jiangxi Province,China. Citrus yellow vein clearing virus （CYVCV） is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the family Alphaflexiviridae. Capsid protein （CP） of CYVCV Chongqing isolate （CYVCV- CQ） was produced using a prokaryotic expression system and used as the immunogen for monoclonal antibody （MAb） production. Four highly specific and sensitive murine MAbs and one polyclonal antibody were prepared in this study. Titers of the four MAbs in ascites fluids ranged from 10-6 to 10-7 as determined by indirect enzyme-linked immunosorbent assay （ELISA）. Three serological assays, including dot enzyme-linked immunosorbent assay （dot-ELISA）, tissue blot-ELISA, and double-antibody sandwich （DAS）-ELISA, were developed for quick and reliable detections of CYVCV in citrus samples. The developed dot-ELISA and DAS-ELISA methods could detect CYVCV in the infected citrus leaf crude extracts diluted at 1：2 560 and 1：10 240 （w/v, g mL^-1）, respectively. The detection result of 125 citrus leaf samples collected from citrus groves in Yunnan Province and Chongqing Municipality of China showed that approximately 36% samples were positive for CYVCV. This virus was, however, not＇detected in any sample collected from Zhejiang or Jiangxi Province, China. |
| Author | LIU Zhen SUNZHU Yuan-ji ZHOU Xue-ping HONG Jian WU Jian-xiang |
| AuthorAffiliation | State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China |
| AuthorAffiliation_xml | – name: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China |
| Author_xml | – sequence: 1 givenname: Zhen surname: LIU fullname: LIU, Zhen – sequence: 2 givenname: Yuan-ji surname: SUNZHU fullname: SUNZHU, Yuan-ji – sequence: 3 givenname: Xue-ping surname: ZHOU fullname: ZHOU, Xue-ping – sequence: 4 givenname: Jian surname: HONG fullname: HONG, Jian – sequence: 5 givenname: Jian-xiang surname: WU fullname: WU, Jian-xiang |
| BookMark | eNqFUcluFDEUbKEgEUI-AanFKTk02H5epsUJjVgiBXEAztbz0o2Hjp3YPROGr8c9k3DgwultVSW76nlzElP0TfOSkteUUPnmKyO96IDS_oLKS0m5Eh170pwyEKwDzsRJ7R8hz5rzUjaEECoEIXJ12vjPKSY7pYhTi3EOJrl9Z7B41xaf05TGYOvJ-dnbOaTYpqFdhzlvS7v305Tu250PsbWTxxzi2O7Cclo2R9CY086XF83TAafizx_qWfP9w_tv60_d9ZePV-t3153lksydpKCctM4Ir4BzxZhgiE4MBoBxiuAcSGsBnTGGgxhsz-mwjHQFcqXgrLk66rqEG32bww3mvU4Y9GGR8qgxz6G-VgtwlQ5GgiGcrYSxDhgKpM71zFpZtS6PWvcYB4yj3qRtrjYV_XuM-5-_tGeEKsIJhYq9OGJvc7rb-jLrm1Bs9QejT9uiWbUcFGFqkX17hNqcSsl-0NUpXKydM4ZJU6KXXPUhV72Eput0yFWzyhb_sB8_-T_eqwfejxTHu5rUX6JUVPXVaAJ_AHPStKs |
| CitedBy_id | crossref_primary_10_1186_s42483_021_00100_2 crossref_primary_10_3389_fmicb_2020_592816 crossref_primary_10_1016_j_cropro_2023_106254 crossref_primary_10_1631_jzus_B2000255 crossref_primary_10_1016_j_jviromet_2020_113837 crossref_primary_10_1007_s00284_024_03614_y crossref_primary_10_1094_PDIS_02_21_0232_RE crossref_primary_10_1007_s12250_019_00186_1 crossref_primary_10_1016_j_hpj_2020_05_001 crossref_primary_10_1007_s00705_018_04123_7 crossref_primary_10_1007_s11033_023_08838_y crossref_primary_10_1007_s12250_018_0024_3 crossref_primary_10_3390_agriculture11090839 crossref_primary_10_1016_j_procbio_2022_09_009 crossref_primary_10_1016_S2095_3119_20_63492_X crossref_primary_10_1094_PDIS_08_22_1970_RE crossref_primary_10_32615_bp_2023_006 crossref_primary_10_1007_s12298_024_01545_3 crossref_primary_10_1007_s10658_023_02653_8 crossref_primary_10_1016_S2095_3119_19_62755_3 crossref_primary_10_3390_ijms23169457 |
| Cites_doi | 10.1007/s00705-015-2423-1 10.1371/journal.pone.0096366 10.1111/j.1439-0434.2008.01498.x 10.1016/j.jviromet.2010.09.027 10.1631/jzus.B1200275 10.1111/j.1467-7652.2007.00270.x 10.1016/j.jviromet.2013.09.013 10.1094/PDIS-04-14-0343-PDN 10.1007/s00705-014-2114-3 10.1094/PHYTO-06-12-0140-R 10.1186/1743-422X-8-228 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W95 ~WA AAYXX CITATION 7S9 L.6 2B. 4A8 92I 93N PSX TCJ DOA |
| DOI | 10.1016/S2095-3119(16)61475-2 |
| DatabaseName | 中文科技期刊数据库 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-农业科学 中文科技期刊数据库- 镜像站点 CrossRef AGRICOLA AGRICOLA - Academic Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | AGRICOLA |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Agriculture |
| DocumentTitleAlternate | Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves |
| EISSN | 2352-3425 |
| EndPage | 891 |
| ExternalDocumentID | oai_doaj_org_article_53dfc93b63b04285bcd32a5a1dd92cc6 zgnykx_e201704013 10_1016_S2095_3119_16_61475_2 671792520 |
| GeographicLocations | China |
| GeographicLocations_xml | – name: China |
| GrantInformation_xml | – fundername: the Special Fund for Agro-scientific Research in the Public Interest,China; the National Basic Research Program of China funderid: (201203076-05); (2014CB138400) |
| GroupedDBID | --M -04 -0D -SD -S~ .~1 0R~ 1B1 1~. 1~5 2B. 2B~ 2RA 4.4 457 4G. 5VR 7-5 8P~ 92G 92I 92L 92M 93N 93Q 9D9 9DD AABNK AABVA AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AATLK AAXUO ABGRD ABMAC ABXDB ABYKQ ACDAQ ACGFS ACRLP ADEZE ADMUD ADQTV AEBSH AEKER AENEX AEQOU AFKWA AFTJW AFUIB AFXIZ AGHFR AGUBO AGYEJ AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLXMC CAJED CAJUS CBWCG CCEZO CHDYS CQIGP EBS EFJIC EFLBG EJD FA0 FDB FIRID FNPLU FYGXN GBLVA GROUPED_DOAJ HZ~ JUIAU KOM M41 MO0 NCXOZ O-L O9- OAUVE OK1 P-8 P-9 PC. Q-- Q-3 Q38 R-D RIG ROL RT4 SDF SDG SES SSA SSZ T5K T8T TCJ TGD U1F U1G U5D U5N W95 ~G- ~WA AAHBH AATTM AAXKI AAYWO AAYXX ABJNI ABWVN ACRPL ACVFH ADCNI ADNMO ADVLN AEIPS AEUPX AFJKZ AFPUW AGCQF AGRNS AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP BNPGV CITATION SSH 7S9 L.6 4A8 PSX EFKBS |
| ID | FETCH-LOGICAL-c460t-6137d6cdb5e734472252aad5fb33241a3dd36cc3adbbb435fc941f3adb1836873 |
| IEDL.DBID | DOA |
| ISSN | 2095-3119 |
| IngestDate | Wed Aug 27 01:14:02 EDT 2025 Thu May 29 03:54:11 EDT 2025 Fri Jul 11 08:51:45 EDT 2025 Tue Jul 01 01:08:40 EDT 2025 Thu Apr 24 23:04:36 EDT 2025 Wed Feb 14 10:03:32 EST 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | monoclonal antibody serological assay Citrus yellow vein clearing virus |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c460t-6137d6cdb5e734472252aad5fb33241a3dd36cc3adbbb435fc941f3adb1836873 |
| Notes | 10-1039/S Citrus yellow vein clearing virus, monoclonal antibody, serological assay Citrus yellow vein clearing virus （CYVCV） is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the family Alphaflexiviridae. Capsid protein （CP） of CYVCV Chongqing isolate （CYVCV- CQ） was produced using a prokaryotic expression system and used as the immunogen for monoclonal antibody （MAb） production. Four highly specific and sensitive murine MAbs and one polyclonal antibody were prepared in this study. Titers of the four MAbs in ascites fluids ranged from 10-6 to 10-7 as determined by indirect enzyme-linked immunosorbent assay （ELISA）. Three serological assays, including dot enzyme-linked immunosorbent assay （dot-ELISA）, tissue blot-ELISA, and double-antibody sandwich （DAS）-ELISA, were developed for quick and reliable detections of CYVCV in citrus samples. The developed dot-ELISA and DAS-ELISA methods could detect CYVCV in the infected citrus leaf crude extracts diluted at 1：2 560 and 1：10 240 （w/v, g mL^-1）, respectively. The detection result of 125 citrus leaf samples collected from citrus groves in Yunnan Province and Chongqing Municipality of China showed that approximately 36% samples were positive for CYVCV. This virus was, however, not＇detected in any sample collected from Zhejiang or Jiangxi Province, China. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://doaj.org/article/53dfc93b63b04285bcd32a5a1dd92cc6 |
| PQID | 2000370276 |
| PQPubID | 24069 |
| PageCount | 8 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_53dfc93b63b04285bcd32a5a1dd92cc6 wanfang_journals_zgnykx_e201704013 proquest_miscellaneous_2000370276 crossref_citationtrail_10_1016_S2095_3119_16_61475_2 crossref_primary_10_1016_S2095_3119_16_61475_2 chongqing_primary_671792520 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2017-04-01 |
| PublicationDateYYYYMMDD | 2017-04-01 |
| PublicationDate_xml | – month: 04 year: 2017 text: 2017-04-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationTitle | Journal of Integrative Agriculture |
| PublicationTitleAlternate | Agricultural Sciences in China |
| PublicationTitle_FL | Journal of Integrative Agriculture |
| PublicationYear | 2017 |
| Publisher | State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China KeAi Communications Co., Ltd |
| Publisher_xml | – name: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China – name: KeAi Communications Co., Ltd |
| References | Wu (10.1016/S2095-3119(16)61475-2_bib13) 2011; 171 Wu (10.1016/S2095-3119(16)61475-2_bib17) 2007; 5 Iftikhar (10.1016/S2095-3119(16)61475-2_bib7) 2010; 48 Önelge (10.1016/S2095-3119(16)61475-2_bib9) 2011; January/February Loconsole (10.1016/S2095-3119(16)61475-2_bib8) 2012; 102 Catara (10.1016/S2095-3119(16)61475-2_bib3) 1992 Chen (10.1016/S2095-3119(16)61475-2_bib5) 2014; 9 Adams (10.1016/S2095-3119(16)61475-2_bib1) 2014; 159 Shang (10.1016/S2095-3119(16)61475-2_bib11) 2011; 8 Grimaldi (10.1016/S2095-3119(16)61475-2_bib6) 1996 Wu (10.1016/S2095-3119(16)61475-2_bib15) 2013; 14 Alshami (10.1016/S2095-3119(16)61475-2_bib2) 2003; 56 Wu (10.1016/S2095-3119(16)61475-2_bib14) 2014; 195 Chen (10.1016/S2095-3119(16)61475-2_bib4) 2014; 98 Song (10.1016/S2095-3119(16)61475-2_bib12) 2015; 160 Önelge (10.1016/S2095-3119(16)61475-2_bib10) 2011 Wu (10.1016/S2095-3119(16)61475-2_bib16) 2009; 15 Zhou (10.1016/S2095-3119(16)61475-2_bib18) 2010 |
| References_xml | – volume: 160 start-page: 1811 year: 2015 ident: 10.1016/S2095-3119(16)61475-2_bib12 article-title: Molecular characterization of a citrus yellow vein clearing virus strain from china publication-title: Archives of Virology doi: 10.1007/s00705-015-2423-1 – volume: 48 start-page: 87 year: 2010 ident: 10.1016/S2095-3119(16)61475-2_bib7 article-title: Effect of environmental factors on Yellow vein clearing virus incidence in lemon publication-title: Journal of Agricultrual Research – start-page: 44 year: 2010 ident: 10.1016/S2095-3119(16)61475-2_bib18 article-title: Current occurrence and research status of graft-transmissible citrus diseases in P.R. China – volume: 9 start-page: e96366 year: 2014 ident: 10.1016/S2095-3119(16)61475-2_bib5 article-title: Development and evaluation of a DAS-ELISA for rapid detection of tembusu virus using monoclonal antibodies against the envelope protein publication-title: PLOS ONE doi: 10.1371/journal.pone.0096366 – volume: 15 start-page: 344 year: 2009 ident: 10.1016/S2095-3119(16)61475-2_bib16 article-title: Monoclonal antibodies against the recombinant nucleocapsid protein of Tomato spotted wilt virus and its application in the virus detection publication-title: Journal of Phytopathology doi: 10.1111/j.1439-0434.2008.01498.x – volume: 171 start-page: 40 year: 2011 ident: 10.1016/S2095-3119(16)61475-2_bib13 article-title: Monoclonal antibody-based triple antibody sandwich-enzyme-linked immunosorbent assay and immunocapture reverse transcription polymerase chain reaction for Odontoglossum ringspot virus detection publication-title: Journal of Virological Methods doi: 10.1016/j.jviromet.2010.09.027 – volume: 14 start-page: 555 year: 2013 ident: 10.1016/S2095-3119(16)61475-2_bib15 article-title: Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China publication-title: Journal of Zhejiang University (Science B) doi: 10.1631/jzus.B1200275 – start-page: 11 year: 2011 ident: 10.1016/S2095-3119(16)61475-2_bib10 article-title: Transmission studies on Citrus yellow vein clearing virus – volume: 5 start-page: 570 year: 2007 ident: 10.1016/S2095-3119(16)61475-2_bib17 article-title: Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens publication-title: Plant Biotechnology Journal doi: 10.1111/j.1467-7652.2007.00270.x – volume: January/February start-page: 22 year: 2011 ident: 10.1016/S2095-3119(16)61475-2_bib9 article-title: Citrus yellow vein clearing virus: A new aphid-transmitted citrus virus publication-title: Citrograph – volume: 195 start-page: 134 year: 2014 ident: 10.1016/S2095-3119(16)61475-2_bib14 article-title: Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors publication-title: Journal of Virological Methods doi: 10.1016/j.jviromet.2013.09.013 – start-page: 343 year: 1996 ident: 10.1016/S2095-3119(16)61475-2_bib6 article-title: Association of a filamentous virus with yellow vein clearing of lemon – volume: 98 start-page: 1747 year: 2014 ident: 10.1016/S2095-3119(16)61475-2_bib4 article-title: First report of citrus yellow vein clearing virus on Lemon in Yunnan, China publication-title: Plant Disease doi: 10.1094/PDIS-04-14-0343-PDN – start-page: 364 year: 1992 ident: 10.1016/S2095-3119(16)61475-2_bib3 article-title: Yellow vein clearing of lemon in Pakistan – volume: 159 start-page: 2831 year: 2014 ident: 10.1016/S2095-3119(16)61475-2_bib1 article-title: Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses publication-title: Archives of Virology doi: 10.1007/s00705-014-2114-3 – volume: 56 start-page: 422 year: 2003 ident: 10.1016/S2095-3119(16)61475-2_bib2 article-title: A hitherto unreported yellow vein clearing disease of citrus in India and its viral etiology publication-title: Indian Phytopathology – volume: 102 start-page: 1168 year: 2012 ident: 10.1016/S2095-3119(16)61475-2_bib8 article-title: Identification and characterization of Citrus yellow vein clearing virus, a putative new member of the genus Mandarivirus publication-title: Phytopathology doi: 10.1094/PHYTO-06-12-0140-R – volume: 8 start-page: 228 year: 2011 ident: 10.1016/S2095-3119(16)61475-2_bib11 article-title: Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus publication-title: Virology Journal doi: 10.1186/1743-422X-8-228 |
| SSID | ssj0001550068 |
| Score | 2.1916623 |
| Snippet | Citrus yellow vein clearing virus （CYVCV） is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the... Citrus yellow vein clearing virus (CYVCV) is considered as the causal agent of Citrus yellow vein clearing disease and be-longs to the genus Mandarivirus in... Citrus yellow vein clearing virus (CYVCV) is considered as the causal agent of Citrus yellow vein clearing disease and belongs to the genus Mandarivirus in the... |
| SourceID | doaj wanfang proquest crossref chongqing |
| SourceType | Open Website Aggregation Database Enrichment Source Index Database Publisher |
| StartPage | 884 |
| SubjectTerms | antigens ascites China Citrus Citrus yellow vein clearing virus coat proteins Dot-ELISA enzyme-linked immunosorbent assay leaves Mandarivirus mice monoclonal antibodies monoclonal antibody orchards polyclonal antibodies serological assay viruses 单克隆抗体 斑点酶联免疫吸附试验 柑桔园 柑橘园 清除病毒 血清学检测 酶联免疫吸附测定 |
| Title | Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves |
| URI | http://lib.cqvip.com/qk/86610A/201704/671792520.html https://www.proquest.com/docview/2000370276 https://d.wanfangdata.com.cn/periodical/zgnykx-e201704013 https://doaj.org/article/53dfc93b63b04285bcd32a5a1dd92cc6 |
| Volume | 16 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LT9wwELYoElJ7QEBbdUtBpuqhPQQ28SPJERAIVYJLi8TNsj1OuipyWnZZtD30t3cmyYblxKVHO7ZjzcMzI3u-YeyT0haDAJklVruQSAhFUhQyJCCDRetXVa7Nrbq80hfX8uuNulkp9UVvwjp44I5wR0pA5UvhtHDk3ivnQWRW2RSgzLxvwbbR5q0EU11-sKLkB6oshz4EHjRp-Zi-c_Rt6Pyc6i9ooHKVZASu8KOJ9W80GU-MVIvl_8QB3XiwsbKxXrFE51tss3ch-XG39W22FuIOe3Vc3_UwGuE1C6iqjb8lL5sj6SaugUVCBgs4itzyvOMQZu1LrMibip-2-Rd8QbcxD3weJpF7qimBG-XzCX2inm5QfdfMw_QNuz4_-356kfQFFRIv9XiGYaLIQXtwKuSE9Ie6nFkLqnIC_arUCgChvRcWnHPoRyHhZVpRExVfF7l4y9ZjE8M7xiG3otSyxKC2kiLIQqcFFITuNfa29OWI7Q7UNL864AyjMXYs8Z_jEZNL-hrfY5FTSYxbMzw6IxYZYpHBVssik43Y4TBtueYzE06IecNgwtJuO1DCTC9h5jkJG7GDJesN6h5dqNgYmvsplfAcixwDexzzsZcJ058BU_OnjoufePpkBFFEYez7_7GbXfaSFuweD31g68j1sId-0cztsxeHf9P9VhH-AQeiB9Y |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Monoclonal+antibody-based+serological+detection+of+Citrus+yellow+vein+clearing+virus+in+citrus+groves&rft.jtitle=Journal+of+Integrative+Agriculture&rft.au=Liu%2C+Zhen&rft.au=SUNZHU%2C+Yuan-ji&rft.au=ZHOU%2C+Xue-ping&rft.au=Hong%2C+Jian&rft.date=2017-04-01&rft.issn=2095-3119&rft.volume=16&rft.issue=4+p.884-891&rft.spage=884&rft.epage=891&rft_id=info:doi/10.1016%2FS2095-3119%2816%2961475-2&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F86610A%2F86610A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzgnykx-e%2Fzgnykx-e.jpg |