Bioassay methods for detection of N-palmitoylbrevetoxin-B2 (BTX-B4)

• Published in: Toxicon (Oxford) Vol. 55; no. 2; pp. 497 - 506
• Main Authors: Bottein, Marie-Yasmine Dechraoui, Fuquay, Jennifer Maucher, Munday, Rex, Selwood, Andrew I., van Ginkel, Roel, Miles, Christopher O., Loader, Jared I., Wilkins, Alistair L., Ramsdell, John S.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2010; Elsevier
• Subjects: Acylation; Animal poisons toxicology. Antivenoms; Animals; Batrachotoxins - analysis; Batrachotoxins - chemical synthesis; Bioassay; Biological and medical sciences; Biological Assay; Brevetoxin; BTX-B2; Cell Line, Tumor; Cell Survival - drug effects; Chromatography, High Pressure Liquid; Enzyme-Linked Immunosorbent Assay; Fatty acid conjugate; Fatty Acids - analysis; Female; Magnetic Resonance Spectroscopy; Mass Spectrometry; Medical sciences; Metabolism; Mice; N-palmitoylbrevetoxin-B2 (BTX-B4); Neurotoxic shellfish poisoning; Plant poisons toxicology; Radioimmunoassay; Toxicology; Brevetoxin; N-palmitoylbrevetoxin-B2 (BTX-B4); Bioassay; Fatty acid conjugate; Neurotoxic shellfish poisoning; Metabolism; BTX-B2; Shellfish; Toxicity; Algae; Lipids; Nervous system; Fatty acids; Toxin; Dinophyta; Plant origin; Neurotoxin; Poisoning; Detection

Brevetoxins (BTXs) are a class of cyclic polyether toxins produced by the dinoflagellate Karenia brevis. These substances are subject to extensive conjugative metabolism in shellfish. BTX-B forms a conjugate with cysteine and is oxidized and reduced to yield BTX-B2, which is further modified by fatt...