Caffeoylquinic Acid Derivatives of Purple Sweet Potato as Modulators of Mitochondrial Function in Mouse Primary Hepatocytes

Owing to their antioxidant properties, caffeoylquinic acid (CQA)-derivatives could potentially improve the impaired metabolism in hepatic cells, however, their effect on mitochondrial function has not been demonstrated yet. Here, we evaluated the impact of three CQA-derivatives extracted from purple...

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Published inMolecules (Basel, Switzerland) Vol. 26; no. 2; p. 319
Main Authors Torres, Andrea, Noriega, Lilia G, Delgadillo-Puga, Claudia, Tovar, Armando R, Navarro-Ocaña, Arturo
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 09.01.2021
MDPI
Subjects
Antioxidants
Caffeoylquinic acid
CQA-derivatives
Diabetes
Diabetes mellitus
Energy demand
Fatty acids
Fatty liver
Glycolysis
hepatic steatosis
Hepatocytes
Kinases
maximal respiration
Metabolic disorders
Metabolic syndrome
Mitochondria
Obesity
Oxidation
Potatoes
purple sweet potato
Respiration
Steatosis
Sweet potatoes
maximal respiration
CQA-derivatives
purple sweet potato
glycolysis
hepatic steatosis
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Summary:Owing to their antioxidant properties, caffeoylquinic acid (CQA)-derivatives could potentially improve the impaired metabolism in hepatic cells, however, their effect on mitochondrial function has not been demonstrated yet. Here, we evaluated the impact of three CQA-derivatives extracted from purple sweet potato, namely 5-CQA, 3,4- and 4,5-diCQA, on mitochondrial activity in primary hepatocytes using an extracellular flux analyzer. Notably, an increase of maximal respiration and spare respiratory capacity were observed when 5-CQA and 3,4-diCQA were added to the system indicating the improved mitochondrial function. Moreover, 3,4-diCQA was shown to considerably increase glycolytic reserve which is a measure of cell capability to respond to an energy demand through glycolysis. Conversely, 4,5-diCQA did not modify mitochondrial activity but increased glycolysis at low concentration in primary hepatocytes. All compounds tested improved cellular capacity to oxidize fatty acids. Overall, our results demonstrated the potential of test CQA-derivatives to modify mitochondrial function in hepatic cells. It is especially relevant in case of dysfunctional mitochondria in hepatocytes linked to hepatic steatosis during obesity, diabetes, and metabolic syndrome.
Bibliography:These authors contributed equally to this work.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26020319