Proteomic techniques identify urine proteins that differentiate patients with interstitial cystitis from asymptomatic control subjects

• Published in: American journal of obstetrics and gynecology Vol. 198; no. 5; pp. 553.e1 - 553.e6
• Main Authors: Canter, Maria P., Graham, Carol A., Heit, Michael H., Blackwell, Linda S., Wilkey, Daniel W., Klein, Jon B., Merchant, Michael L.
• Format: Journal Article
• Language: English
• Published: United States Mosby, Inc 01.05.2008
• Subjects: Adolescent; Adult; Aged; Alpha-Globulins - urine; Biomarkers - urine; Blood Proteins - urine; Cystitis, Interstitial - diagnosis; Cystitis, Interstitial - urine; Diagnosis, Differential; Electrophoresis, Polyacrylamide Gel; Female; Glycoproteins - urine; Humans; interstitial cystitis; Kininogens - urine; Male; mass spectrometry; Middle Aged; Mucoproteins - urine; Obstetrics and Gynecology; Protein Isoforms; Proteinase Inhibitory Proteins, Secretory - urine; Proteins - analysis; proteomic technique; Proteomics; Quality of Life; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Urine - chemistry; Uromodulin; mass spectrometry; interstitial cystitis; proteomic technique
• ISSN: 0002-9378; 1097-6868; 1097-6868
• DOI: 10.1016/j.ajog.2008.01.052

The purpose of this study was to identify differences in urine proteins between patients with interstitial cystitis (IC) and asymptomatic control (AC) subjects with the use of proteomic techniques. Nine patients with IC and their age-, race-, and sex-matched AC subjects volunteered a urine specimen. Urine proteins were separated with the use of 2-dimensional polyacrylamide gels. Differing proteins underwent digestion and matrix-assisted laser desorption ionization-time of flight mass spectrometry. Computer-assisted data analysis was used to identify the corresponding protein. Differences in urine protein responses between patients with IC and AC subjects were evaluated by the Mann-Whitney U test to account for the nonnormal frequency distribution of the parameter estimate or chi-square when data were bimodal. Four proteins differed significantly between patients with IC and AC subjects. The AC subjects had a greater concentration of a uromodulin ( P = .019) and two kininogens ( P = .023, .046). The patients with IC had a greater concentration of inter-alpha-trypsin inhibitor heavy chain H4 ( P = .019). These urine protein isoforms may be biomarkers for IC.