An Enzymatic 2‐Step Cofactor and Co‐Product Recycling Cascade towards a Chiral 1,2‐Diol. Part II: Catalytically Active Inclusion Bodies

• Published in: Advanced synthesis & catalysis Vol. 361; no. 11; pp. 2616 - 2626
• Main Authors: Jäger, Vera D., Piqueray, Maja, Seide, Selina, Pohl, Martina, Wiechert, Wolfgang, Jaeger, Karl‐Erich, Krauss, Ulrich
• Format: Journal Article
• Language: English
• Published: Heidelberg Wiley Subscription Services, Inc 06.06.2019
• Subjects: Alcohol; Alcohol dehydrogenase; Benzaldehyde; Benzyl alcohol; biocatalysis; Cascade chemical reactions; Conversion; enzyme immobilization; Enzymes; inclusion bodies; Nicotinamide; Optimization; Oxidation; protein co-localization; Pseudomonas fluorescens; Recycling; Reduction; Stability; Substrates; synthetic reaction cascades; Thiamine

Optimal performance of multi‐step enzymatic one‐pot cascades requires a facile balance between enzymatic activity and stability of multiple enzymes under the employed reaction conditions. We here describe the optimization of an exemplary two‐step one‐pot recycling cascade utilizing the thiamine diph...