A new genetic tool to improve immune‐compromised mouse models: Derivation and CRISPR/Cas9‐mediated targeting of NRG embryonic stem cell lines

• Published in: Genesis (New York, N.Y. : 2000) Vol. 56; no. 9; pp. e23238 - n/a
• Main Authors: Martin Gonzalez, Javier, Baudet, Aurélie, Abelechian, Sahar, Bonderup, Kasper, d'Altri, Teresa, Porse, Bo, Brakebusch, Cord, Juliusson, Gunnar, Cammenga, Jörg
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.09.2018; Wiley Subscription Services, Inc
• Subjects: Animal models; Animals; Basic Medicine; Breeding; Cell Line; Cell lines; Clinical Medicine; CRISPR; CRISPR-Cas Systems; CRISPR/Cas9; Derivation; Diabetes mellitus; Embryo cells; Embryogenesis; Embryonic Stem Cells; Embryos; ES cell derivation; Fertilization in Vitro; Fetuses; Gata-2; GATA2 Transcription Factor - genetics; Gene Targeting; Genomes; Hematologi; Hematology; hematopoiesis; Hematopoietic stem cells; Immunocompromised Host - genetics; immunodeficient mice; Implantation; In vitro fertilization; Klinisk medicin; Medical and Health Sciences; Medical Genetics; Medicin och hälsovetenskap; Medicinsk genetik; Medicinska och farmaceutiska grundvetenskaper; Mice; Mice, Inbred NOD - genetics; Mice, Inbred NOD - immunology; Models, Biological; Mutation; NRG; Nuclease; Phenotypes; Pluripotency; RAG1 protein; Rodents; Stem cell transplantation; Stem cells; Xenografts; Xenotransplantation; immunodeficient mice; Gata-2; NRG; hematopoiesis; CRISPR/Cas9; ES cell derivation; CRISPR; Cas9

Summary Development of human hematopoietic stem cells and differentiation of embryonic stem (ES) cells/induced pluripotent stem (iPS) cells to hematopoietic stem cells are poorly understood. NOD (Non‐obese diabetic)‐derived mouse strains, such as NSG (NOD‐Scid‐il2Rg) or NRG (NOD‐Rag1‐il2Rg), are the...