Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus

• Published in: Nature chemical biology Vol. 9; no. 4; pp. 277 - 283
• Main Authors: Ouchi, Takuya, Tomita, Takeo, Horie, Akira, Yoshida, Ayako, Takahashi, Kento, Nishida, Hiromi, Lassak, Kerstin, Taka, Hikari, Mineki, Reiko, Fujimura, Tsutomu, Kosono, Saori, Nishiyama, Chiharu, Masui, Ryoji, Kuramitsu, Seiki, Albers, Sonja-Verena, Kuzuyama, Tomohisa, Nishiyama, Makoto
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.04.2013; Nature Publishing Group
• Subjects: 2-Aminoadipic Acid - metabolism; 631/114/1386; 631/1647/2258/1266; 631/326/26/2527; 631/92/1643; 631/92/458; 631/92/60; 631/92/612/1222; Amino acids; Archaeal Proteins - chemistry; Archaeal Proteins - genetics; Archaeal Proteins - metabolism; Arginine - biosynthesis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Biosynthesis; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Catalytic Domain; Cell Biology; Chemistry; Chemistry/Food Science; Crystallography, X-Ray; Escherichia coli - genetics; Evolution, Molecular; Evolutionary biology; Gene Duplication; Glutamic Acid - metabolism; Lysine - biosynthesis; Models, Molecular; Ornithine - metabolism; Phylogeny; Protein Binding; Protein Interaction Domains and Motifs; Proteins; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Substrate Specificity; Sulfolobus acidocaldarius; Sulfolobus acidocaldarius - genetics; Sulfolobus acidocaldarius - metabolism; Thermus; Thermus - genetics; Thermus - metabolism

One pathway for lysine biosynthesis uses a carrier protein, LysW, to protect the substrate. LysW is now shown to mediate entry of a second substrate into the same metabolic pathway, with structural and biochemical evidence identifying an amino acid motif that determines substrate specificity. LysW h...