Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR

• Published in: Molecular breeding Vol. 15; no. 4; pp. 349 - 366
• Main Authors: Merdinoglu, D, Butterlin, G, Bevilacqua, L, Chiquet, V, Adam-Blondon, A.F, Decroocq, S
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.05.2005; Springer Verlag
• Subjects: Alleles; Biochemistry, Molecular Biology; Deoxyribonucleic acid; DNA; DNA libraries; DNA primers; Fluorescence; Genetic analysis; Genetic markers; genomic libraries; grapes; Heterozygosity; Inserts; Life Sciences; Loci; Markers; microsatellite repeats; Microsatellites; Molecular biology; molecular sequence data; multiplex polymerase chain reaction; Multiplexing; nucleotide sequences; Plant biology; Polymerase chain reaction; Primers; Vitis vinifera; SEQUENCES SIMPLES REPETEES; MULTIPLEX PCR; MICROSATELLITE; POLYMORPHISM; GRAPEVINE; SIMPLE SEQUENCE REPEATS

Despite their numerous advantages, the use of microsatellites as genetic markers could be limited because of the low number of loci that can be simultaneously analysed per experiment. To increase the information per simple sequence repeat (SSR) assay in the grapevine, we developed a large set of new...